LOCUS       sequence-16614-e        1385 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1385)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1995
REFERENCE   2  (bases 1 to 1385)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pPD93_48
FEATURES             Location/Qualifiers
     source          1..1385
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             357..377
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(420..448)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 gctgcggtcg actctagagg atccccaatt cttgagactt ccactatctt ctctggcacc
       61 ctccactacg cgtaggcgtc tctcgcttcg tgtattcccg ggaagccggg ttcccgtctc
      121 tcccgccgct gccgctgccg cacacagctt tacacctcgt agaatcccca aagaggggcg
      181 tggcttgcgg gtgccaacat cctcctgccg aggaagaagc aggcactcat cactcgcatc
      241 atcaacctcg ggattggcca aaggacccaa aggtatgttt cgaatgatac taacataaca
      301 tagaacattt tcaggaggac ccttggaggg taccgagctc agaaaaaatg actgctccaa
      361 agaagaagcg taaggtaccg gtagaaaaaa tgagtaaagg agaagaactt ttcactggag
      421 ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg gcacaaattt tctgtcagtg
      481 gagagggtga aggtgatgca acatacggaa aacttaccct taaatttatt tgcactactg
      541 gaaaactacc tgttccatgg gtaagtttaa acatatatat actaactaac cctgattatt
      601 taaattttca gccaacactt gtcactactt tctgttatgg tgttcaatgc ttctcgagat
      661 acccagatca tatgaaacgg catgactttt tcaagagtgc catgcccgaa ggttatgtac
      721 aggaaagaac tatatttttc aaagatgacg ggaactacaa gacacgtaag tttaaacagt
      781 tcggtactaa ctaaccatac atatttaaat tttcaggtgc tgaagtcaag tttgaaggtg
      841 atacccttgt taatagaatc gagttaaaag gtattgattt taaagaagat ggaaacattc
      901 ttggacacaa attggaatac aactataact cacacaatgt atacatcatg gcagacaaac
      961 naaaagaatg gaatccaagt tgtangtttt aacatgattt tactaactaa ctaatctgan
     1021 tttaattttc cgaacttcna aattagacnc acattgaana tggangcgtt canctagcaa
     1081 accttttcna caaaatactc cattggngan ggcctgtcct ttnacaaaan accatnactn
     1141 gtccnacaat ctgcccttnc aaanatccna cgaaaaaaaa aacccntggc cctnttgant
     1201 tgnaacccgn nggganaacn nnggggggag anataaaaaa ncttngnaaa ttcccgnngg
     1261 cngggcctca atacanttgg ngggnnaaaa aangggccnc tccccaaaan tnaccggctn
     1321 ccccacggaa atnttttttc ccnccncngn ccngaataaa aaatttccan cnagnttttc
     1381 cgncc
//
