LOCUS       sequence-166141-         940 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Use to synthesize riboprobes for in situ hybridization (ISH) probe 
            for En2. Not for gene expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 940)
  AUTHORS   Parrilla M, Chang I, Degl'Innocenti A, Omura M
  TITLE     Expression of homeobox genes in the mouse olfactory epithelium.
  JOURNAL   J Comp Neurol. 2016 Oct 1;524(14):2713-39. doi: 10.1002/cne.24051. 
            Epub 2016 Jun 19.
  PUBMED    27243442
REFERENCE   2  (bases 1 to 940)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/cne.24051";
            journalName: "J Comp Neurol"; date: "2016-10-1- 1"; volume: "524"; 
            issue: "14"; pages: "2713-39"
COMMENT     Sequence Label: pISH-En2
FEATURES             Location/Qualifiers
     source          1..940
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      7..16
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      11..20
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(752..770)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(753..770)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(788..804)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(788..804)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(801..823)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    812..828
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(836..842,843..860,861..866))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    881..902
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ctcccggccg ccatggcggc cgcgggaatt cgattgaaga tgattccaac tcgctctgcc
       61 aggccccaag gcctatctcg gtgagggctt ttgggtcagg gctagctgtc ccggcagcaa
      121 agccttcaag ccttcaggac acactcccac tgctccccag gccccaacgc tggccacggt
      181 ggcgggagag ttggcgctgg caggtcgcta cggggctgtg gcggacaggg gtcatcgaca
      241 cagacgcaga cacacggtgg ctgccagcac gggcctctga gatggttgag aagagaggct
      301 gcgtaggaag caggcggaga ggttccggga gagctgctgg cctgcaagga ctttctcaga
      361 cctcagcaaa gaactgcgcc ctctctcccc accagagctc tccatcacca ctctggctgg
      421 cgcttcctac agtgctgtcc gagccagcac aaagcaagcg gcagagccca cagaccaaat
      481 aggagctatt tattcggttc acagtcctgt gaaatgcagc aataaaaatc tttggacttc
      541 agcaagttgt tttaaatttt ttttctttcg ggggaagaag tgtccaatgt cccataatgg
      601 aatttaaggg attaaaaaaa gggagaaaag aaaaatgaaa gaaagatctg aaaccctaaa
      661 cagtcccctt taatcactag tgaattcgcg gccgcctgca ggtcgaccat atgggagagc
      721 tcccaacgcg ttggatgcat agcttgagta ttctatagtg tcacctaaat agcttggcgt
      781 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
      841 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
      901 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
//
