LOCUS       sequence-166143-        1048 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Use to synthesize riboprobes for in situ hybridization (ISH) probe 
            for En2. Not for gene expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Parrilla M, Chang I, Degl'Innocenti A, Omura M
  TITLE     Expression of homeobox genes in the mouse olfactory epithelium.
  JOURNAL   J Comp Neurol. 2016 Oct 1;524(14):2713-39. doi: 10.1002/cne.24051. 
            Epub 2016 Jun 19.
  PUBMED    27243442
REFERENCE   2  (bases 1 to 1048)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/cne.24051";
            journalName: "J Comp Neurol"; date: "2016-10-1- 1"; volume: "524"; 
            issue: "14"; pages: "2713-39"
COMMENT     Sequence Label: pISH-En2
FEATURES             Location/Qualifiers
     source          1..1048
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      717..726
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      721..730
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(760..779)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(761..779)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(786..803)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(786..802)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(795..817)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 atccaacgcg ttgggagctc tcccatatgg tcgacctgca ggcggccgcg aattcactag
       61 tgattaaagg ggactgttta gggtttcaga tctttctttc atttttcttt tctccctttt
      121 tttaatccct taaattccat tatgggacat tggacacttc ttcccccgaa agaaaaaaaa
      181 tttaaaacaa cttgctgaag tccaaagatt tttattgctg catttcacag gactgtgaac
      241 cgaataaata gctcctattt ggtctgtggg ctctgccgct tgctttgtgc tggctcggac
      301 agcactgtag gaagcgccag ccagagtggt gatggagagc tctggtgggg agagagggcg
      361 cagttctttg ctgaggtctg agaaagtcct tgcaggccag cagctctccc ggaacctctc
      421 cgcctgcttc ctacgcagcc tctcttctca accatctcag aggcccgtgc tggcagccac
      481 cgtgtgtctg cgtctgtgtc gatgacccct gtccgccaca gccccgtagc gacctgccag
      541 cgccaactct cccgccaccg tggccagcgt tggggcctgg ggagcagtgg gagtgtgtcc
      601 tgaaggcttg aaggctttgc tgccgggaca gctagccctg acccaaaagc cctcaccgag
      661 ataggccttg gggcctggca gagcgagttg gaatcatctt caatcgaatt cccgcggccg
      721 ccatggcggc cgggagcatg cgacgtcggg cccaattcgc cctatagtga gtcgtattac
      781 aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
      841 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
      901 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggacgcgccc tgtagcggcg
      961 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     1021 tagcgcccgc tcctttcgct ttcttccc
//
