LOCUS       sequence-166152-        3843 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Use to synthesize riboprobes for in situ hybridization (ISH) probe 
            for Meis3. Not for gene expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3843)
  AUTHORS   Parrilla M, Chang I, Degl'Innocenti A, Omura M
  TITLE     Expression of homeobox genes in the mouse olfactory epithelium.
  JOURNAL   J Comp Neurol. 2016 Oct 1;524(14):2713-39. doi: 10.1002/cne.24051. 
            Epub 2016 Jun 19.
  PUBMED    27243442
REFERENCE   2  (bases 1 to 3843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/cne.24051";
            journalName: "J Comp Neurol"; date: "2016-10-1- 1"; volume: "524"; 
            issue: "14"; pages: "2713-39"
COMMENT     Sequence Label: pISH-Meis3
FEATURES             Location/Qualifiers
     source          1..3843
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      37..46
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(968..986)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(969..986)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1004..1020)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1004..1020)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1017..1039)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1028..1044
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1052..1058,1059..1076,1077..1082))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1097..1118
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1235..1252)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1406..1994)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1486..1505)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2165..2956,2957..3025))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2788..2807
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3026..3130)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3208..3663)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3345..3366)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3557..3576
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3789..3811
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3803..3820
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3804..3820
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(3827..3843,1..3)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        join(3827..3843,1..2)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggcgaattg ggcccgacgt cgcatgctcc cggccgccat ggcggccgcg ggaattcgat
       61 tcttggccac tcggagaccc tggcacccgg cggggacctc ggggacctcc tgccgcgatg
      121 agctccaggc cgtgacctcc cgagcccagg acaggcggcg ccggctcatg gcccggaggt
      181 atgatgagct gcgccactac ccaggcatta cagagcacac gacagccctg gccagctttt
      241 cagaggcagc gccttcagta cctagagccc ctgggcccta cacccacacc ggccacccca
      301 gctccaagct ccaggcttgg acagcgacag cttgaagaga gagaaggatg acatctatgg
      361 acaccccctc ttcccactct tggccctggt ctttgagaag tgtgagctgg ctacatgctc
      421 gccccgtgat ggggcctcgg ctgggctggg ttcaccccca ggtggtgacg tctgctcctc
      481 tgactccttc aatgaggata ttgctgcctt tgctaagcag atccgctccg agaggccgct
      541 gttctcttcc aacccggagc tggacaacct gatggtccag gccatccagg tactccggtt
      601 ccacctgctg gagctggaga aggggaagat gcccatagac ctggtgattg aggatcggga
      661 tggtagttgc agggaggatc ttgaggacta cgcagcctcc tgccccagcc tcccagacca
      721 gaatactaca tggatcagag accatgagga cagtgggtct gtacatttgg ggaccccagg
      781 tccatccagc ggaggcctgg cctcccagag tggggacaac tcaagtgacc aaggagatgg
      841 gctggacaca agcgtagcct ctcccagctc cgcaggagag gatgaggaat cactagtgaa
      901 ttcgcggccg cctgcaggtc gaccatatgg gagagctccc aacgcgttgg atgcatagct
      961 tgagtattct atagtgtcac ctaaatagct tggcgtaatc atggtcatag ctgtttcctg
     1021 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     1081 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     1141 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     1201 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     1261 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     1321 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     1381 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     1441 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     1501 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     1561 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     1621 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     1681 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     1741 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     1801 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     1861 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     1921 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     1981 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     2041 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     2101 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     2161 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     2221 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     2281 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     2341 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     2401 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     2461 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     2521 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     2581 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     2641 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     2701 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     2761 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     2821 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     2881 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     2941 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     3001 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     3061 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     3121 gggttccgcg cacatttccc cgaaaagtgc cacctgatgc ggtgtgaaat accgcacaga
     3181 tgcgtaagga gaaaataccg catcaggaaa ttgtaagcgt taatattttg ttaaaattcg
     3241 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc
     3301 cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga
     3361 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg
     3421 atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag
     3481 cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga
     3541 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg
     3601 tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg
     3661 cgtccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
     3721 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
     3781 agggttttcc cagtcacgac gttgtaaaac gacggccagt gaattgtaat acgactcact
     3841 ata
//
