LOCUS       sequence-166155-        1021 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Use to synthesize riboprobes for in situ hybridization (ISH) probe 
            for Msx1. Not for gene expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Parrilla M, Chang I, Degl'Innocenti A, Omura M
  TITLE     Expression of homeobox genes in the mouse olfactory epithelium.
  JOURNAL   J Comp Neurol. 2016 Oct 1;524(14):2713-39. doi: 10.1002/cne.24051. 
            Epub 2016 Jun 19.
  PUBMED    27243442
REFERENCE   2  (bases 1 to 1021)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/cne.24051";
            journalName: "J Comp Neurol"; date: "2016-10-1- 1"; volume: "524"; 
            issue: "14"; pages: "2713-39"
COMMENT     Sequence Label: pISH-Msx1
FEATURES             Location/Qualifiers
     source          1..1021
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      468..477
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      472..481
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(511..530)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(512..530)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(537..554)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(537..553)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(546..568)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(781..800)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     991..1012
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ccaacgcgtt gggagctctc ccatatggtc gacctgcagg cggccgcgaa ttcactagtg
       61 atttaccacc tggtcccttc tcttaaaccc cttgctacac acttcctcct ggttgtcgct
      121 tcctaaacct tcctcatctg accccttctg ggaagaaaaa gaatggtcgg aagtgtctag
      181 gtttttcgag aaaaatctag atttacatgc gcaagttata aatgtggaaa ctaagggtgc
      241 agaggccaag agatttatcc gtggtcccca gcagaattag aggctgaagg agaccagagg
      301 ccaaaaggac tagaggccat gagactccat cagctgcttc cggtcctgaa accaggcagg
      361 acttgcacag agaaattgct aatcggtgct taaagagatg agcccagccc tatagaaagc
      421 aaggagccca gctccttcca ctgtcaaact ctaatcgaat tcccgcggcc gccatggcgg
      481 ccgggagcat gcgacgtcgg gcccaattcg ccctatagtg agtcgtatta caattcactg
      541 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
      601 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
      661 tcccaacagt tgcgcagcct gaatggcgaa tggacgcgcc ctgtagcggc gcattaagcg
      721 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
      781 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
      841 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
      901 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
      961 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     1021 t
//
