LOCUS       sequence-166156-         991 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Use to synthesize riboprobes for in situ hybridization (ISH) probe 
            for Msx1. Not for gene expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Parrilla M, Chang I, Degl'Innocenti A, Omura M
  TITLE     Expression of homeobox genes in the mouse olfactory epithelium.
  JOURNAL   J Comp Neurol. 2016 Oct 1;524(14):2713-39. doi: 10.1002/cne.24051. 
            Epub 2016 Jun 19.
  PUBMED    27243442
REFERENCE   2  (bases 1 to 991)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/cne.24051";
            journalName: "J Comp Neurol"; date: "2016-10-1- 1"; volume: "524"; 
            issue: "14"; pages: "2713-39"
COMMENT     Sequence Label: pISH-Msx1
FEATURES             Location/Qualifiers
     source          1..991
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      7..16
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      11..20
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(505..523)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(506..523)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(541..557)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(541..557)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(554..576)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    565..581
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(589..595,596..613,614..619))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    634..655
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(772..789)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ctcccggccg ccatggcggc cgcgggaatt cgattagagt ttgacagtgg aaggagctgg
       61 gctccttgct ttctataggg ctgggctcat ctctttaagc accgattagc aatttctctg
      121 tgcaagtcct gcctggtttc aggaccggaa gcagctgatg gagtctcatg gcctctagtc
      181 cttttggcct ctggtctcct tcagcctcta attctgctgg ggaccacgga taaatctctt
      241 ggcctctgca cccttagttt ccacatttat aacttgcgca tgtaaatcta gatttttctc
      301 gaaaaaccta gacacttccg accattcttt ttcttcccag aaggggtcag atgaggaagg
      361 tttaggaagc gacaaccagg aggaagtgtg tagcaagggg tttaagagaa gggaccaggt
      421 ggtaaatcac tagtgaattc gcggccgcct gcaggtcgac catatgggag agctcccaac
      481 gcgttggatg catagcttga gtattctata gtgtcaccta aatagcttgg cgtaatcatg
      541 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
      601 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
      661 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
      721 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
      781 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
      841 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
      901 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
      961 ccctgacgag catcacaaaa atcgacgctc a
//
