LOCUS       sequence-166157-        1011 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TG2 biosensor that allows for quantitative assessment of TG2 
            conformational changes in live cells using FRET.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Caron NS, Munsie LN, Keillor JW, Truant R
  TITLE     Using FLIM-FRET to measure conformational changes of 
            transglutaminase type 2 in live cells.
  JOURNAL   PLoS One. 2012;7(8):e44159. doi: 10.1371/journal.pone.0044159. Epub 
            2012 Aug 31.
  PUBMED    22952912
REFERENCE   2  (bases 1 to 1011)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(8):e44159. doi"; pages: " 
            10.1371/journal.pone.0044159. Epub 2012 Aug 31"
COMMENT     Sequence Label: mCer-TG2-YFP
FEATURES             Location/Qualifiers
     source          1..1011
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     12..33
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
       61 gacgagctgt acaagtccgg actcagatct ggtggcggag ggatggccga ggagctggtc
      121 ttagagaggt gtgatctgga gctggagacc aatggccgag accaccacac ggccgacctg
      181 tgccgggaga agctggtggt gcgacggggc cagcccttct ggctgaccct gcactttgag
      241 ggccgcaact acgaggccag tgtagacagt ctcaccttca gtgtcgtgac cggcccagcc
      301 cctagccagg aggccgggac caaggcccgt tttccactaa gagatgctgt ggaggagggt
      361 gactggacag ccaccgtggt ggaccagcaa gactgcaccc tctcgctgca gctcaccacc
      421 ccggccaacg cccccatcgg cctgtatcgc ctcagcctgg aggcctccac tggctaccag
      481 ggatccagct ttgtgctggg ccacttcatt ttgctcttca acacctggtg cccagcggat
      541 gctgtgtacc tggactcgga agaggagcgg caggagtatg tcctcaccca gcagggcttt
      601 atctaccagg gctcggccaa gttcatcaag aacatacctt ggaattttgg gcagtttgaa
      661 gatgggatcc tagacatctg cctgatcctt ctagatgtca accccaagtt cctgaagaac
      721 gccggccgtg actgctcccg ccgcagcagc cccgtctacg tgggccgggt gggtagtggc
      781 atggtcaact gcaacgatga ccagggtgtg ctgctgggac gctgggacaa caactacggg
      841 gacggcgtca gccccatgtc ctggatcggc agcgtggaca tcctgcggcg ctggaagaac
      901 cacggctgcc agcgcgtcaa gtatggccag tgctgggtct tcgccgccgt ggcctgcaca
      961 gtgctgaggt gcctaggcat ccctacccgc gtcgtgacca actacaactc g
//
