LOCUS       sequence-166158-        6814 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6814)
  AUTHORS   Mani SA, Guo W, Liao MJ, Eaton EN, Ayyanan A, Zhou AY, Brooks M, 
            Reinhard F, Zhang CC, Shipitsin M, Campbell LL, Polyak K, Brisken C,
            Yang J, Weinberg RA
  TITLE     The epithelial-mesenchymal transition generates cells with 
            properties of stem cells.
  JOURNAL   Cell. 2008 May 16. 133(4):704-15.
  PUBMED    18485877
REFERENCE   2  (bases 1 to 6814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2008 
            May 16. 133(4):704-15."
COMMENT     Sequence Label: pWZL Blast Snail ER
FEATURES             Location/Qualifiers
     source          1..6814
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             8..477
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    542..741
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             942..1358
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1288..1310
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1326..1342
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    3231..3692
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3287..3304)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3514..3533
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     LTR             4308..4777
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(4907..4924)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5078..5666)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5158..5177)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5826..6617,6618..6686))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6449..6468
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6687..6791)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgcagcctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca
       61 gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt
      121 tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag
      181 tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc
      241 ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga
      301 gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc
      361 gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc
      421 tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcattt
      481 gggggctcgt ccgggatcgg gagacccctg cccagggacc accgacccac caccgggagg
      541 caagctggcc agcaacttat ctgtgtctgt ccgattgtct agtgtctatg actgatttta
      601 tgcgcctgcg tcggtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa
      661 ctgacgagtt ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc
      721 gtttttgtgg cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg
      781 tggttctggt aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt
      841 cggtttggaa ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt
      901 gtctctgtct gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc
      961 ccttaagttt gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg
     1021 tagatgtcaa gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg
     1081 tcggatggcc gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg
     1141 tcttttcacc tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag
     1201 ccttggcttt tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc
     1261 ctcttcctcc atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat
     1321 cctcccttta tccagccctc actccttctc taggcgccgg ccggatccat gccgcgctct
     1381 ttcctcgtca ggaagccctc cgaccccaat cggaagccta actacagcga gctgcaggac
     1441 tctaatccag agtttacctt ccagcagccc tacgaccagg cccacctgct ggcagccatc
     1501 ccacctccgg agatcctcaa ccccaccgcc tcgctgccaa tgctcatctg ggactctgtc
     1561 ctggcgcccc aagcccagcc aattgcctgg gcctcccttc ggctccagga gagtcccagg
     1621 gtggcagagc tgacctccct gtcagatgag gacagtggga aaggctccca gccccccagc
     1681 ccaccctcac cggctccttc gtccttctcc tctacttcag tctcttcctt ggaggccgag
     1741 gcctatgctg ccttcccagg cttgggccaa gtgcccaagc agctggccca gctctctgag
     1801 gccaaggatc tccaggctcg aaaggccttc aactgcaaat actgcaacaa ggaatacctc
     1861 agcctgggtg ccctcaagat gcacatccga agccacacgc tgccctgcgt ctgcggaacc
     1921 tgcgggaagg ccttctctag gccctggctg ctacaaggcc atgtccggac ccacactggc
     1981 gagaagccct tctcctgtcc ccactgcagc cgtgccttcg ctgaccgctc caacctgcgg
     2041 gcccacctcc agacccactc agatgtcaag aagtaccagt gccaggcgtg tgctcggacc
     2101 ttctcccgaa tgtccctgct ccacaagcac caagagtccg gctgctcagg atgtccccgc
     2161 gcggatccac gaaatgaaat gggtgcttca ggagacatga gggctgccaa cctttggcca
     2221 agccctcttg tgattaagca cactaagaag aatagccctg ccttgtcctt gacagctgac
     2281 cagatggtca gtgccttgtt ggatgctgaa ccgcccatga tctattctga atatgatcct
     2341 tctagaccct tcagtgaagc ctcaatgatg ggcttattga ccaacctagc agatagggag
     2401 ctggttcata tgatcaactg ggcaaagaga gtgccaggct ttggggactt gaatctccat
     2461 gatcaggtcc accttctcga gtgtgcctgg ctggagattc tgatgattgg tctcgtctgg
     2521 cgctccatgg aacacccggg gaagctcctg tttgctccta acttgctcct ggacaggaat
     2581 caaggtaaat gtgtggaagg catggtggag atctttgaca tgttgcttgc tacgtcaagt
     2641 cggttccgca tgatgaacct gcagggtgaa gagtttgtgt gcctcaaatc catcattttg
     2701 cttaattccg gagtgtacac gtttctgtcc agcaccttga agtctctgga agagaaggac
     2761 cacatccacc gtgtcctgga caagatcaca gacactttga tccacctgat ggccaaagct
     2821 ggcctgactc tgcagcagca gcatcgccgc ctagctcagc tccttctcat tctttcccat
     2881 atccggcata tgagtaacaa acgcatggag catctctaca acatgaaatg caagaacgtg
     2941 gtacccctct atgacctgct cctggagatg ttggatgccc accgccttca tgccccagcc
     3001 agtcgcatgg gagtgccccc agaggagccc agccagaccc agctggccac caccagctcc
     3061 acttcagcac attccttaca aacctactac atacccccgg aagcagaggg cttccccaac
     3121 acgatctgag agctcctact agtgaattca ctcgagatac gtaaaacaat tgaagatcta
     3181 gtcgaccaat tccggttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg
     3241 aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga
     3301 atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa
     3361 acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc
     3421 tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac
     3481 gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag
     3541 gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtgc
     3601 acatgcttta catgtgttta gtcgaggtta aaaaacgtct aggccccccg aaccacgggg
     3661 acgtggtttt cctttgaaaa acacgatgat aataccatga aaacatttaa catttctcaa
     3721 caggatctag aattagtaga agtagcgaca gagaagatta caatgcttta tgaggataat
     3781 aaacatcatg tgggagcggc aattcgtacg aaaacaggag aaatcatttc ggcagtacat
     3841 attgaagcgt atataggacg agtaactgtt tgtgcagaag ccattgcgat tggtagtgca
     3901 gtttcgaatg gacaaaagga ttttgacacg attgtagctg ttagacaccc ttattctgac
     3961 gaagtagata gaagtattcg agtggtaagt ccttgtggta tgtgtaggga gttgatttca
     4021 gactatgcac cagattgttt tgtgttaata gaaatgaatg gcaagttagt caaaactacg
     4081 attgaagaac tcattccact caaatatacc cgaaattaaa agttttacca taccaagctc
     4141 atcgataaaa taaaagattt tatttagtct ccagaaaaag gggggaatga aagaccccac
     4201 ctgtaggttt ggcaagctag cttaagtaac gccattttgc aaggcatgga aaaatacata
     4261 actgagaata gagaagttca gatcaaggtc aggaacagat ggaacagctg aatatgggcc
     4321 aaacaggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagatggaac
     4381 agctgaatat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg gctcagggcc
     4441 aagaacagat ggtccccaga tgcggtccag ccctcagcag tttctagaga accatcagat
     4501 gtttccaggg tgccccaagg acctgaaatg accctgtgcc ttatttgaac taaccaatca
     4561 gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga gctcaataaa agagcccaca
     4621 acccctcact cggggcgcca gtcctccgat tgactgagtc gcccgggtac ccgtgtatcc
     4681 aataaaccct cttgcagttg catccgactt gtggtctcgc tgttccttgg gagggtctcc
     4741 tctgagtgat tgactacccg tcagcggggg tctttcacat gcagcatgta tcaaaattaa
     4801 tttggttttt tttcttaagt atttacatta aatggccata gttgcattaa tgaatcggcc
     4861 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     4921 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     4981 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     5041 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     5101 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     5161 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     5221 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     5281 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     5341 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     5401 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     5461 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     5521 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     5581 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     5641 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     5701 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     5761 tcacctagat ccttttgcgg ccgcaaatca atctaaagta tatatgagta aacttggtct
     5821 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     5881 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     5941 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     6001 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     6061 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     6121 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     6181 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     6241 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     6301 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     6361 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     6421 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     6481 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     6541 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     6601 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     6661 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     6721 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     6781 ggggttccgc gcacatttcc ccgaaaagtg ccac
//
