LOCUS       sequence-166159-        6640 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6640)
  AUTHORS   Mani SA, Guo W, Liao MJ, Eaton EN, Ayyanan A, Zhou AY, Brooks M, 
            Reinhard F, Zhang CC, Shipitsin M, Campbell LL, Polyak K, Brisken C,
            Yang J, Weinberg RA
  TITLE     The epithelial-mesenchymal transition generates cells with 
            properties of stem cells.
  JOURNAL   Cell. 2008 May 16. 133(4):704-15.
  PUBMED    18485877
REFERENCE   2  (bases 1 to 6640)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2008 
            May 16. 133(4):704-15."
COMMENT     Sequence Label: pWZL Blast Twist ER
FEATURES             Location/Qualifiers
     source          1..6640
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             8..477
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    542..741
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             942..1358
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1288..1310
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1326..1342
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    3057..3518
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3113..3130)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3340..3359
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     LTR             4134..4603
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(4733..4750)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4904..5492)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4984..5003)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5652..6443,6444..6512))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6513..6617)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgcagcctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca
       61 gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt
      121 tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag
      181 tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc
      241 ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga
      301 gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc
      361 gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc
      421 tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcattt
      481 gggggctcgt ccgggatcgg gagacccctg cccagggacc accgacccac caccgggagg
      541 caagctggcc agcaacttat ctgtgtctgt ccgattgtct agtgtctatg actgatttta
      601 tgcgcctgcg tcggtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa
      661 ctgacgagtt ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc
      721 gtttttgtgg cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg
      781 tggttctggt aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt
      841 cggtttggaa ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt
      901 gtctctgtct gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc
      961 ccttaagttt gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg
     1021 tagatgtcaa gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg
     1081 tcggatggcc gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg
     1141 tcttttcacc tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag
     1201 ccttggcttt tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc
     1261 ctcttcctcc atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat
     1321 cctcccttta tccagccctc actccttctc taggcgccgg ccggatccat gatgcaggac
     1381 gtgtccagct cgccagtctc tccggccgac gacagcctga gcaacagcga ggaggagccg
     1441 gaccggcagc agccggcgag cggcaagcgc ggggctcgca agagacgcag cagtcggcgc
     1501 agcgcgggcg gcagcgcggg gcccggcggg gccacgggcg ggggcatcgg aggcggcgac
     1561 gagccaggca gcccggccca gggcaagcgc ggcaagaaat ctgcgggcgg aggcggcggc
     1621 ggcggcgcgg gcggaggtgg tggcggcggc ggcggcagca gcagcggggg cgggagcccg
     1681 cagtcgtacg aggagctgca gacccagcgg gtcatggcta acgtgcggga gcgccagcgc
     1741 acgcagtcgc tgaacgaggc gttcgccgcc ctgcgcaaga tcatccccac gctgccctcg
     1801 gacaagctga gcaagattca gaccctcaaa ctggcggcca ggtacatcga cttcctgtac
     1861 caggtcctgc agagcgacga gctggactcc aagatggcaa gctgcagcta tgtggcccac
     1921 gagcggctca gctacgcctt ctccgtctgg aggatggagg gggcctggtc catgtccgcg
     1981 tcccacgcgg atccacgaaa tgaaatgggt gcttcaggag acatgagggc tgccaacctt
     2041 tggccaagcc ctcttgtgat taagcacact aagaagaata gccctgcctt gtccttgaca
     2101 gctgaccaga tggtcagtgc cttgttggat gctgaaccgc ccatgatcta ttctgaatat
     2161 gatccttcta gacccttcag tgaagcctca atgatgggct tattgaccaa cctagcagat
     2221 agggagctgg ttcatatgat caactgggca aagagagtgc caggctttgg ggacttgaat
     2281 ctccatgatc aggtccacct tctcgagtgt gcctggctgg agattctgat gattggtctc
     2341 gtctggcgct ccatggaaca cccggggaag ctcctgtttg ctcctaactt gctcctggac
     2401 aggaatcaag gtaaatgtgt ggaaggcatg gtggagatct ttgacatgtt gcttgctacg
     2461 tcaagtcggt tccgcatgat gaacctgcag ggtgaagagt ttgtgtgcct caaatccatc
     2521 attttgctta attccggagt gtacacgttt ctgtccagca ccttgaagtc tctggaagag
     2581 aaggaccaca tccaccgtgt cctggacaag atcacagaca ctttgatcca cctgatggcc
     2641 aaagctggcc tgactctgca gcagcagcat cgccgcctag ctcagctcct tctcattctt
     2701 tcccatatcc ggcatatgag taacaaacgc atggagcatc tctacaacat gaaatgcaag
     2761 aacgtggtac ccctctatga cctgctcctg gagatgttgg atgcccaccg ccttcatgcc
     2821 ccagccagtc gcatgggagt gcccccagag gagcccagcc agacccagct ggccaccacc
     2881 agctccactt cagcacattc cttacaaacc tactacatac ccccggaagc agagggcttc
     2941 cccaacacga tctgagagct cctactagtg aattcactcg agatacgtaa aacaattgaa
     3001 gatctagtcg accaattccg gttattttcc accatattgc cgtcttttgg caatgtgagg
     3061 gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc
     3121 aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga
     3181 agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg
     3241 tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag
     3301 tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc
     3361 aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct
     3421 cggtgcacat gctttacatg tgtttagtcg aggttaaaaa acgtctaggc cccccgaacc
     3481 acggggacgt ggttttcctt tgaaaaacac gatgataata ccatgaaaac atttaacatt
     3541 tctcaacagg atctagaatt agtagaagta gcgacagaga agattacaat gctttatgag
     3601 gataataaac atcatgtggg agcggcaatt cgtacgaaaa caggagaaat catttcggca
     3661 gtacatattg aagcgtatat aggacgagta actgtttgtg cagaagccat tgcgattggt
     3721 agtgcagttt cgaatggaca aaaggatttt gacacgattg tagctgttag acacccttat
     3781 tctgacgaag tagatagaag tattcgagtg gtaagtcctt gtggtatgtg tagggagttg
     3841 atttcagact atgcaccaga ttgttttgtg ttaatagaaa tgaatggcaa gttagtcaaa
     3901 actacgattg aagaactcat tccactcaaa tatacccgaa attaaaagtt ttaccatacc
     3961 aagctcatcg ataaaataaa agattttatt tagtctccag aaaaaggggg gaatgaaaga
     4021 ccccacctgt aggtttggca agctagctta agtaacgcca ttttgcaagg catggaaaaa
     4081 tacataactg agaatagaga agttcagatc aaggtcagga acagatggaa cagctgaata
     4141 tgggccaaac aggatatctg tggtaagcag ttcctgcccc ggctcagggc caagaacaga
     4201 tggaacagct gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc
     4261 agggccaaga acagatggtc cccagatgcg gtccagccct cagcagtttc tagagaacca
     4321 tcagatgttt ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac
     4381 caatcagttc gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag
     4441 cccacaaccc ctcactcggg gcgccagtcc tccgattgac tgagtcgccc gggtacccgt
     4501 gtatccaata aaccctcttg cagttgcatc cgacttgtgg tctcgctgtt ccttgggagg
     4561 gtctcctctg agtgattgac tacccgtcag cgggggtctt tcacatgcag catgtatcaa
     4621 aattaatttg gttttttttc ttaagtattt acattaaatg gccatagttg cattaatgaa
     4681 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     4741 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     4801 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     4861 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     4921 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     4981 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     5041 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     5101 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     5161 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     5221 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     5281 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     5341 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     5401 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     5461 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     5521 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     5581 ggatcttcac ctagatcctt ttgcggccgc aaatcaatct aaagtatata tgagtaaact
     5641 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     5701 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     5761 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     5821 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     5881 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     5941 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     6001 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     6061 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     6121 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     6181 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     6241 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     6301 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     6361 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     6421 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     6481 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     6541 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     6601 caaatagggg ttccgcgcac atttccccga aaagtgccac
//
