LOCUS       sequence-166164-         939 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Use to synthesize riboprobes for in situ hybridization (ISH) probe 
            for Msx2. Not for gene expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 939)
  AUTHORS   Parrilla M, Chang I, Degl'Innocenti A, Omura M
  TITLE     Expression of homeobox genes in the mouse olfactory epithelium.
  JOURNAL   J Comp Neurol. 2016 Oct 1;524(14):2713-39. doi: 10.1002/cne.24051. 
            Epub 2016 Jun 19.
  PUBMED    27243442
REFERENCE   2  (bases 1 to 939)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/cne.24051";
            journalName: "J Comp Neurol"; date: "2016-10-1- 1"; volume: "524"; 
            issue: "14"; pages: "2713-39"
COMMENT     Sequence Label: pISH-Msx2
FEATURES             Location/Qualifiers
     source          1..939
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      7..16
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      11..20
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(745..763)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(746..763)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(781..797)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(781..797)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(794..816)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    805..821
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(829..835,836..853,854..859))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    874..895
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ctcccggccg ccatggcggc cgcgggaatt cgattttgac ctgggtctct gtaaggttca
       61 gagagctgga gaactcggcc cgctctgcta tggacaggta ctgtttctgg cggaacttgc
      121 gctccaaggc tagaagctgg gatgtggtga agggtgtgcg tggcttccgg ttggtcttgt
      181 gtttcctcag ggtgcaggtg gtggggctca tatgtctggg cggcggggag tatctgccgg
      241 gctcctgtat ccacggtgct ccgtcttcgg aattttccga cttgaccgag gcggtctcga
      301 agggcttgac gagaggcccg ggactgtgag cgtcccggac gccgtgtccc ggcagcagca
      361 gcggccgcag gacagcgcca gccgaggcgc agtcgggtgg caccgcgggc gattccttgg
      421 gcggcttctt gtcggacatg agcgcctcca cgctgaaggg caggctggag accttgaccc
      481 tgcgttcctc tgcgctgccc tcggctcctc caggcccggg acccgggccg gccagtaccg
      541 cggggccctc ctcatccgac gaaaacaagt caccgccttt agtcggagaa gccatgactt
      601 tctgcgccgc gtagctgtcg gcgcgcgact ccgctggcaa ctctcaagcg aagactccaa
      661 ctttaatcac tagtgaattc gcggccgcct gcaggtcgac catatgggag agctcccaac
      721 gcgttggatg catagcttga gtattctata gtgtcaccta aatagcttgg cgtaatcatg
      781 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
      841 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
      901 gttgcgctca ctgcccgctt tccagtcggg aaacctgtc
//
