LOCUS       sequence-166165-         978 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Use to synthesize riboprobes for in situ hybridization (ISH) probe 
            for Msx2. Not for gene expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Parrilla M, Chang I, Degl'Innocenti A, Omura M
  TITLE     Expression of homeobox genes in the mouse olfactory epithelium.
  JOURNAL   J Comp Neurol. 2016 Oct 1;524(14):2713-39. doi: 10.1002/cne.24051. 
            Epub 2016 Jun 19.
  PUBMED    27243442
REFERENCE   2  (bases 1 to 978)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/cne.24051";
            journalName: "J Comp Neurol"; date: "2016-10-1- 1"; volume: "524"; 
            issue: "14"; pages: "2713-39"
COMMENT     Sequence Label: pISH-Msx2
FEATURES             Location/Qualifiers
     source          1..978
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      708..717
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      712..721
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(751..770)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(752..770)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(777..794)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(777..793)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(786..808)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 ccaacgcgtt gggagctctc ccatatggtc gacctgcagg cggccgcgaa ttcactagtg
       61 attaaagttg gagtcttcgc ttgagagttg ccagcggagt cgcgcgccga cagctacgcg
      121 gcgcagaaag tcatggcttc tccgactaaa ggcggtgact tgttttcgtc ggatgaggag
      181 ggccccgcgg tactggccgg cccgggtccc gggcctggag gagccgaggg cagcgcagag
      241 gaacgcaggg tcaaggtctc cagcctgccc ttcagcgtgg aggcgctcat gtccgacaag
      301 aagccgccca aggaatcgcc cgcggtgcca cccgactgcg cctcggctgg cgctgtcctg
      361 cggccgctgc tgctgccggg acacggcgtc cgggacgctc acagtcccgg gcctctcgtc
      421 aagcccttcg agaccgcctc ggtcaagtcg gaaaattccg aagacggagc accgtggata
      481 caggagcccg gcagatactc cccgccgccc agacatatga gccccaccac ctgcaccctg
      541 aggaaacaca agaccaaccg gaagccacgc acacccttca ccacatccca gcttctagcc
      601 ttggagcgca agttccgcca gaaacagtac ctgtccatag cagagcgggc cgagttctcc
      661 agctctctga accttacaga gacccaggtc aaaatcgaat tcccgcggcc gccatggcgg
      721 ccgggagcat gcgacgtcgg gcccaattcg ccctatagtg agtcgtatta caattcactg
      781 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
      841 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
      901 tcccaacagt tgcgcagcct gaatggcgaa tggacgcgcc ctgtagcggc gcattaagcg
      961 cggcgggtgt ggtggtta
//
