LOCUS       sequence-166167-         966 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Use to synthesize riboprobes for in situ hybridization (ISH) probe 
            for Otx1. Not for gene expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Parrilla M, Chang I, Degl'Innocenti A, Omura M
  TITLE     Expression of homeobox genes in the mouse olfactory epithelium.
  JOURNAL   J Comp Neurol. 2016 Oct 1;524(14):2713-39. doi: 10.1002/cne.24051. 
            Epub 2016 Jun 19.
  PUBMED    27243442
REFERENCE   2  (bases 1 to 966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/cne.24051";
            journalName: "J Comp Neurol"; date: "2016-10-1- 1"; volume: "524"; 
            issue: "14"; pages: "2713-39"
COMMENT     Sequence Label: pISH-Otx1
FEATURES             Location/Qualifiers
     source          1..966
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      7..16
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      11..20
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(635..653)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(636..653)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(671..687)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(671..687)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(684..706)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    695..711
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(719..725,726..743,744..749))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    764..785
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(902..919)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ctcccggccg ccatggcggc cgcgggaatt cgattcgaga gctcgggttc agagagcagc
       61 ggccagttca cgccgcccgc cgtatctagc tctgcttctt cgtctagctc agcgtccagt
      121 gcctccgcca accccgcggc tgctgcggcc gcgggcctgg gtgggaaccc agtggcagca
      181 gcgtcctctc tgagcacgcc tactgcttcg tccatctgga gcccggcctc catctcgccg
      241 ggctcagcgc cgacatccgt atcggtgcca gagccactgg ccgctccgag caacgcctcg
      301 tgcatgcagc gctcggtagc cgcaggtgcg gccactgccg cagcctccta ccctatgtcc
      361 tatggccagg gcggaagcta tggtcaggga taccccgcgc cctcctcttc ttactttggc
      421 ggtgtagact gcagctccta cctagcgccc atgcactctc atcaccaccc gcaccagctt
      481 agccccatgg caccctcctc catggctggc catcatcacc atcaccctca tgcgcaccac
      541 ccactgagcc aatcaatcac tagtgaattc gcggccgcct gcaggtcgac catatgggag
      601 agctcccaac gcgttggatg catagcttga gtattctata gtgtcaccta aatagcttgg
      661 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
      721 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
      781 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
      841 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
      901 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
      961 caaagg
//
