LOCUS       sequence-166168-         967 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Use to synthesize riboprobes for in situ hybridization (ISH) probe 
            for Otx1. Not for gene expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Parrilla M, Chang I, Degl'Innocenti A, Omura M
  TITLE     Expression of homeobox genes in the mouse olfactory epithelium.
  JOURNAL   J Comp Neurol. 2016 Oct 1;524(14):2713-39. doi: 10.1002/cne.24051. 
            Epub 2016 Jun 19.
  PUBMED    27243442
REFERENCE   2  (bases 1 to 967)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/cne.24051";
            journalName: "J Comp Neurol"; date: "2016-10-1- 1"; volume: "524"; 
            issue: "14"; pages: "2713-39"
COMMENT     Sequence Label: pISH-Otx1
FEATURES             Location/Qualifiers
     source          1..967
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      599..608
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      603..612
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(642..661)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(643..661)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(668..685)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(668..684)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(677..699)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(912..931)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tccaacgcgt tgggagctct cccatatggt cgacctgcag gcggccgcga attcactagt
       61 gattgattgg ctcagtgggt ggtgcgcatg agggtgatgg tgatgatggc cagccatgga
      121 ggagggtgcc atggggctaa gctggtgcgg gtggtgatga gagtgcatgg gcgctaggta
      181 ggagctgcag tctacaccgc caaagtaaga agaggagggc gcggggtatc cctgaccata
      241 gcttccgccc tggccatagg acatagggta ggaggctgcg gcagtggccg cacctgcggc
      301 taccgagcgc tgcatgcacg aggcgttgct cggagcggcc agtggctctg gcaccgatac
      361 ggatgtcggc gctgagcccg gcgagatgga ggccgggctc cagatggacg aagcagtagg
      421 cgtgctcaga gaggacgctg ctgccactgg gttcccaccc aggcccgcgg ccgcagcagc
      481 cgcggggttg gcggaggcac tggacgctga gctagacgaa gaagcagagc tagatacggc
      541 gggcggcgtg aactggccgc tgctctctga acccgagctc tcgaatcgaa ttcccgcggc
      601 cgccatggcg gccgggagca tgcgacgtcg ggcccaattc gccctatagt gagtcgtatt
      661 acaattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
      721 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
      781 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggacgcgc cctgtagcgg
      841 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
      901 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
      961 ccgtcaa
//
