LOCUS       sequence-166175-         969 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Use to synthesize riboprobes for in situ hybridization (ISH) probe 
            for Pbx1. Not for gene expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 969)
  AUTHORS   Parrilla M, Chang I, Degl'Innocenti A, Omura M
  TITLE     Expression of homeobox genes in the mouse olfactory epithelium.
  JOURNAL   J Comp Neurol. 2016 Oct 1;524(14):2713-39. doi: 10.1002/cne.24051. 
            Epub 2016 Jun 19.
  PUBMED    27243442
REFERENCE   2  (bases 1 to 969)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/cne.24051";
            journalName: "J Comp Neurol"; date: "2016-10-1- 1"; volume: "524"; 
            issue: "14"; pages: "2713-39"
COMMENT     Sequence Label: pISH-Pbx1
FEATURES             Location/Qualifiers
     source          1..969
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      369..378
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      373..382
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(412..431)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(413..431)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(438..455)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(438..454)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(447..469)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      595..953
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(682..701)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     892..913
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 caacgcgttg ggagctctcc catatggtcg acctgcaggc ggccgcgaat tcactagtga
       61 ttacaactaa tcgcataggg gagagaacat ggttgcaggt atagcatctc ccacatgtca
      121 gtcactgtgg gaggggttgc acatgtatta ttttgactaa tccctaccac atctccaaga
      181 ggaattccta tcctctaata aataatgaaa attgagagtc cgaaatattt agtaacttgc
      241 ccatggccac atagataaaa aaaacctaga gcctattttc aggcttagat gtacctaact
      301 aactccatag tctatgcttt gttactaacc cagacctgcc agtgagagaa gtcaatcgaa
      361 ttcccgcggc cgccatggcg gccgggagca tgcgacgtcg ggcccaattc gccctatagt
      421 gagtcgtatt acaattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
      481 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
      541 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggacgcgc
      601 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
      661 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
      721 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
      781 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
      841 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
      901 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
      961 ttttgccga
//
