LOCUS       sequence-166177-         882 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Use to synthesize riboprobes for in situ hybridization (ISH) probe 
            for Phtf1. Not for gene expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Parrilla M, Chang I, Degl'Innocenti A, Omura M
  TITLE     Expression of homeobox genes in the mouse olfactory epithelium.
  JOURNAL   J Comp Neurol. 2016 Oct 1;524(14):2713-39. doi: 10.1002/cne.24051. 
            Epub 2016 Jun 19.
  PUBMED    27243442
REFERENCE   2  (bases 1 to 882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/cne.24051";
            journalName: "J Comp Neurol"; date: "2016-10-1- 1"; volume: "524"; 
            issue: "14"; pages: "2713-39"
COMMENT     Sequence Label: pISH-Phtf1
FEATURES             Location/Qualifiers
     source          1..882
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      7..16
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      11..20
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(434..452)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(435..452)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(470..486)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(470..486)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(483..505)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    494..510
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(518..524,525..542,543..548))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    563..584
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(701..718)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ctcccggccg ccatggcggc cgcgggaatt cgattcagtt gcgtcatgct tcgggcacct
       61 accatggtta cccaagagac tatttacagt aagtataact agcacagtac tggaggctcc
      121 aggataaatg tcctgactca ggaatagtcc gagtctttgt gaggcaggct ttagtttgct
      181 gactattcac gttgcttgag aaccatgaaa gtgcaggttt ttaacacagg ccccggggaa
      241 aggaggtgcc gaagctccat tcgttagacg gggctgagtg gagagactga tgcatgtgag
      301 cctgcaggtg gggccactgg tttctgtggg ccatctttgt cctcagtggg ggaaatcact
      361 agtgaattcg cggccgcctg caggtcgacc atatgggaga gctcccaacg cgttggatgc
      421 atagcttgag tattctatag tgtcacctaa atagcttggc gtaatcatgg tcatagctgt
      481 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
      541 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
      601 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
      661 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
      721 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
      781 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
      841 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gc
//
