LOCUS       sequence-166178-        1050 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Use to synthesize riboprobes for in situ hybridization (ISH) probe 
            for Phtf1. Not for gene expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Parrilla M, Chang I, Degl'Innocenti A, Omura M
  TITLE     Expression of homeobox genes in the mouse olfactory epithelium.
  JOURNAL   J Comp Neurol. 2016 Oct 1;524(14):2713-39. doi: 10.1002/cne.24051. 
            Epub 2016 Jun 19.
  PUBMED    27243442
REFERENCE   2  (bases 1 to 1050)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/cne.24051";
            journalName: "J Comp Neurol"; date: "2016-10-1- 1"; volume: "524"; 
            issue: "14"; pages: "2713-39"
COMMENT     Sequence Label: pISH-Phtf1
FEATURES             Location/Qualifiers
     source          1..1050
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      398..407
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      402..411
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(441..460)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(442..460)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(467..484)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(467..483)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(476..498)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      624..1004
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(711..730)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     921..942
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 tccaacgcgt tgggagctct cccatatggt cgacctgcag gcggccgcga attcactagt
       61 gatttccccc actgaggaca aagatggccc acagaaacca gtggccccac ctgcaggctc
      121 acatgcatca gtctctccac tcagccccgt ctaacgaatg gagcttcggc acctcctttc
      181 cccggggcct gtgttaaaaa cctgcacttt catggttctc aagcaacgtg aatagtcagc
      241 aaactaaagc ctgcctcaca aagactcgga ctattcctga gtcaggacat ttatcctgga
      301 gcctccagta ctgtgctagt tatacttact gtaaatagtc tcttgggtaa ccatggtagg
      361 tgcccgaagc atgacgcaac tgaatcgaat tcccgcggcc gccatggcgg ccgggagcat
      421 gcgacgtcgg gcccaattcg ccctatagtg agtcgtatta caattcactg gccgtcgttt
      481 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
      541 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
      601 tgcgcagcct gaatggcgaa tggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
      661 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
      721 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
      781 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
      841 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
      901 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
      961 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     1021 tgagctgatt taacaaaaat ttaacgcgaa
//
