LOCUS       sequence-166181-         987 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Use to synthesize riboprobes for in situ hybridization (ISH) probe 
            for Pitx1. Not for gene expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 987)
  AUTHORS   Parrilla M, Chang I, Degl'Innocenti A, Omura M
  TITLE     Expression of homeobox genes in the mouse olfactory epithelium.
  JOURNAL   J Comp Neurol. 2016 Oct 1;524(14):2713-39. doi: 10.1002/cne.24051. 
            Epub 2016 Jun 19.
  PUBMED    27243442
REFERENCE   2  (bases 1 to 987)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/cne.24051";
            journalName: "J Comp Neurol"; date: "2016-10-1- 1"; volume: "524"; 
            issue: "14"; pages: "2713-39"
COMMENT     Sequence Label: pISH-Pitx1
FEATURES             Location/Qualifiers
     source          1..987
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      591..600
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      595..604
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(634..653)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(635..653)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(660..677)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(660..676)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(669..691)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(904..923)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 atccaacgcg ttgggagctc tcccatatgg tcgacctgca ggcggccgcg aattcactag
       61 tgattcctcc cttggcttgc tttcttttct tttcattcat tgggggacag agcgggggtc
      121 tggaaaaagc aaacacaaaa ccaacctgga ggcggggtgg ggaggaaggg gctgcgcaga
      181 ggtgaggtcc gaggagctgc gagcgccccg cgtgctcccg cgcgctccct tctgtgagcc
      241 ttgctggccg gcggccccgg ggaccaggcg gggcggtcag ctgttgtact ggcaagcgtt
      301 gagccccgac gccggaccct gcagaccgcc atagccaaac gacgagtgct gcttggactt
      361 gagccgcagg ctggcaaggc tcgagttgca cgtgtcccgg tagacgctgt agggcgaggc
      421 tggggtgccg taggggcagg cgcccggcga catggccgag ttgagcgagg agccggtgag
      481 gttgttgatg ttgttgaggc ccgagttggg catgccgggc acggcgcccg ggcccatgct
      541 ggacggcatg gtcatggaag agatggagct gggggaatcg aattcccgcg gccgccatgg
      601 cggccgggag catgcgacgt cgggcccaat tcgccctata gtgagtcgta ttacaattca
      661 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
      721 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
      781 ccttcccaac agttgcgcag cctgaatggc gaatggacgc gccctgtagc ggcgcattaa
      841 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
      901 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
      961 ctctaaatcg ggggctccct ttagggt
//
