LOCUS       sequence-166187-         949 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 949)
  AUTHORS   Ayaydin F, Dasso M
  TITLE     Distinct in vivo dynamics of vertebrate SUMO paralogues.
  JOURNAL   Mol Biol Cell. 2004 Dec . 15(12):5208-18.
  PUBMED    15456902
REFERENCE   2  (bases 1 to 949)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2004 Dec . 15(12):5208-18."
COMMENT     Sequence Label: pEYFP SUMO-1
FEATURES             Location/Qualifiers
     source          1..949
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(542..793)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(584..605)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt
       61 ttttaaagca agtaaaacct ctacaaatgt ggtatggctg attatgatca gttatctaga
      121 tccggtggat cccgggcccg cggtaccgtc gagcagtcag tcacgatgaa ttgcccgatc
      181 cactagtaac ggccgccagt gtgctggaat tcggcttatt ctaaactgtt gaatgacccc
      241 ccgtttgttc ctgataaact tcaatcacat cttcttcctc cattcccagt tcttttggag
      301 tatgattatc agcaattctc tgaccctcaa agagaaacct gagtgaattc attggaacac
      361 cctgtctttg acagtatgat tctttgagtt tcttgagatg tgttgtcatt ttcactttga
      421 agtgaatctc actgctatcc tgtccaatga ctttgagttt aatatattca ccttccttct
      481 tatcccccaa gtcctcagtt gaaggttttg cctcctggtc agacatggat ctgagtccgg
      541 acttgtacag ctcgtccatg ccgagagtga tcccggcggc ggtcacgaac tccagcagga
      601 ccatgtgatc gcgcttctcg ttggggtctt tgctcagggc ggactggtag ctcaggtagt
      661 ggttgtcggg cagcagcacg gggccgtcgc cgatgggggt gttctgctgg tagtggtcgg
      721 cgagctgcac gctgccgtcc tcgatgttgt ggcggatctt gaagttcacc ttgatgccgt
      781 tcttctgctt gtcggccatg atatagacgt tgtggctgtt gtagttgtac tccagcttgt
      841 gccccaggat gttgccgtcc tccttgaagt cgatgccctt cagctcgatg cggttcacca
      901 gggtgtcgcc ctcgaacttc acctcggcgc gggtcttgta gttgccgtc
//
