LOCUS       sequence-166188-         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Gray JT, Zolotukhin S
  TITLE     Design and Construction of Functional AAV Vectors.
  JOURNAL   Methods Mol Biol. 2011;807:25-46.
  PUBMED    22034025
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2011"; volume: "807"; pages: "25-46"
COMMENT     Sequence Label: pAAV-GFP
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     213..234
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      276..285
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     intron          363..838
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
ORIGIN
        1 gtagccttcg ggcatggcgg acttgaagaa gtcgtgctgc ttcatgtggt cggggtagcg
       61 gctgaagcac tgcacgccgt aggtcagggt ggtcacgagg gtgggccagg gcacgggcag
      121 cttgccggtg gtgcagatga acttcagggt cagcttgccg taggtggcat cgccctcgcc
      181 ctcgccggac acgctgaact tgtggccgtt tacgtcgccg tccagctcga ccaggatggg
      241 caccaccccg gtgaacagct cctcgccctt gctcaccatg gtggcgaccg gtggatcccg
      301 ggcccgcgga attctttgcc aaagtgatgg gccagcacac agaccagcac gttgcccagg
      361 agctgtggga ggaagataag aggtatgaac atgattagca aaagggccta gcttggactc
      421 agaataatcc agccttatcc caaccataaa ataaaagcag aatggtagct ggattgtagc
      481 tgctattagc aatatgaaac ctcttacatc agttacaatt tatatgcaga aatatttata
      541 tgcagaaata ttgctattgc cttaacccag aaattatcac tgttattctt tagaatggtg
      601 caaagaggca tgatacattg tatcattatt gccctgaaag aaagagatta gggaaagtat
      661 tagaaataag ataaacaaaa aagtatatta aaagaagaaa gcatttttta aaattacaaa
      721 tgcaaaatta ccctgatttg gtcaatatgt gtaccctgtt acttctcccc ttcctatgac
      781 atgaacttaa ccatagaaaa gaaggggaaa gaaaacatca agggtcccat agactcaccc
//
