LOCUS       sequence-166189-         832 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Arabinose inducible lambda red and anhydrotetracycline inducible 
            sgRNA expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Reisch CR, Prather KL
  TITLE     The no-SCAR (Scarless Cas9 Assisted Recombineering) system for 
            genome editing in Escherichia coli.
  JOURNAL   Sci Rep. 2015 Oct 14;5:15096. doi: 10.1038/srep15096.
  PUBMED    26463009
REFERENCE   2  (bases 1 to 832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-10-14"; volume: "5:15096. doi"; pages: " 
            10.1038/srep15096"
COMMENT     Sequence Label: pKDsgRNA-ack
FEATURES             Location/Qualifiers
     source          1..832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(7..24)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(40..69)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     misc_RNA        complement(90..165)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    196..214
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    221..239
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 aactcaatga tgatgatgat gatggtcgac ggcgctattc agatcctctt ctgagatgag
       61 tttttgttcg ggcccaagct tcaaaaaaag caccgactcg gtgccacttt ttcaagttga
      121 taacggacta gccttatttt aacttgctat ttctagctct aaaacaaagc cgcttcctgt
      181 ttattgtgct cagtatctct atcactgata gggatgtcaa tctctatcac tgatagggaa
      241 gaattccaga aatcatcctt agcgaaagct gataatctca tgaccaaaat cccttaacgt
      301 gagttttcgt tccactgagc gtcagacccc gttgatgata ccgctgcctt actgggtgca
      361 ttagccagtc tgaatgacct gtcacgggat aatccgaagt ggtcagactg gaaaatcaga
      421 gggcaggaac tgctgaacag caaaaagtca gatagcacca catagcagac ccgccataaa
      481 acgccctgag aagcccgtga cgggcttttc ttgtattatg ggtagtttcc ttgcatgaat
      541 ccataaaagg cgcctgtagt gccatttacc cccattcact gccagagccg tgagcgcagc
      601 gaactgaatg tcacgaaaaa gacagcgact caggtgcctg atggtcggag acaaaaggaa
      661 tattcagcga tttgcccgat tgcggccgca accgagcttg cgagggtgct acttaagcct
      721 ttagggtttt aaggtctgtt ttgtagagga gcaaacagcg tttgcgacat ccttttgtaa
      781 tactgcggaa ctgactaaag tagtgagtta tacacagggc tgggatctat tc
//
