LOCUS       sequence-16619-e        1326 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1326)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1995
REFERENCE   2  (bases 1 to 1326)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pPD95_02
FEATURES             Location/Qualifiers
     source          1..1326
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(70..98)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     846..866
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(917..933)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(917..933)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(965..984)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(966..984)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tacctcctaa gggacaaaag ctgggtaccg gtagaaaaaa tgagtaaagg agaagaactt
       61 ttcactggag ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg gcacaaattt
      121 tctgtcagtg gagagggtga aggtgatgca acatacggaa aacttaccct taaatttatt
      181 tgcactactg gaaaactacc tgttccatgg gtaagtttaa acatatatat actaactaac
      241 cctgattatt taaattttca gccaacactt gtcactactt tctgttatgg tgttcaatgc
      301 ttctcgagat acccagatca tatgaaacgg catgactttt tcaagagtgc catgcccgaa
      361 ggttatgtac aggaaagaac tatatttttc aaagatgacg ggaactacaa gacacgtaag
      421 tttaaacagt tcggtactaa ctaaccatac atatttaaat tttcaggtgc tgaagtcaag
      481 tttgaaggtg atacccttgt taatagaatc gagttaaaag gtattgattt taaagaagat
      541 ggaaacattc ttggacacaa attggaatac aactataact cacacaatgt atacatcatg
      601 gcagacaaac aaaagaatgg aatcaaagtt gtaagtttaa acatgatttt actaactaac
      661 taatctgatt taaattttca gaacttcaaa attagacaca acattgaaga tggaagcgtt
      721 caactagcag accattatca acaaaatact ccaattggcg atggccctgt ccttttacca
      781 gacaaccatt acctgtccac acaatctgcc ctttcgaaag atcccaacga aaagagagac
      841 cacatggtcc ttcttgagtt tgtaacagct gctgggatta cacatggcat ggatgaacta
      901 tacnaatggc ccgggggatc cactagttct agagcggccg ccaccgcggt ggagctccca
      961 ttcgccctat agtgagtcgt attacncgcg ctcnctggcc gtcgttttac aacgtcnnga
     1021 ctgggaaaaa ccctggcgtt accccactta atccccctgg cagcccatcc cccctttccc
     1081 ccagctgggg gtaaannncg aanaggnccc gcccccgatc gccccttccc aanagttggc
     1141 ccncccntga ntgggnaaag ggggaccccc ccngtaaccg gnccatnann cccgggcggg
     1201 gggggggggt taccccccan nggggaccnc ttaccctggg ccgggnccnt angnccccnc
     1261 cccntttcnc ttttcncccn tncctttccn cccccngttt gnccgggttt tcccnccnna
     1321 gnccta
//
