LOCUS       sequence-166195-        1026 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Use to synthesize riboprobes for in situ hybridization (ISH) probe 
            for Prrx1. Not for gene expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Parrilla M, Chang I, Degl'Innocenti A, Omura M
  TITLE     Expression of homeobox genes in the mouse olfactory epithelium.
  JOURNAL   J Comp Neurol. 2016 Oct 1;524(14):2713-39. doi: 10.1002/cne.24051. 
            Epub 2016 Jun 19.
  PUBMED    27243442
REFERENCE   2  (bases 1 to 1026)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/cne.24051";
            journalName: "J Comp Neurol"; date: "2016-10-1- 1"; volume: "524"; 
            issue: "14"; pages: "2713-39"
COMMENT     Sequence Label: pISH-Prrx1
FEATURES             Location/Qualifiers
     source          1..1026
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      582..591
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      586..595
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(625..644)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(626..644)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(651..668)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(651..667)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(660..682)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(895..914)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tccaacgcgt tgggagctct cccatatggt cgacctgcag gcggccgcga attcactagt
       61 gatttaacat agacatggcc acaagggttg ggccaatgaa gacccagaac atacatgggt
      121 ttctctcatt cttacagaca cagataccta tcgttgagat cagagctgct tcaggctaag
      181 ttcacaaggg gtgtgtagaa aacttccaaa tgcatctacg acgtttctct gaagaactta
      241 cttggacctg agagagcatt tagatgaggc tttgggattt agtggcacct atcaagtgtg
      301 tcaaagcact tgtgtttcac aaatgtggca tttctcatct ttatctgggc ccaattccct
      361 gtaaccagtt ttaaatccct ccatgggtca agcattaact ttaaaaagat acacagatgt
      421 cattcaatag aaaaatgaat gtgaacatag ttcttctggg caggatatat aatagccttg
      481 acttgggttt ctccaaggtt taaagaaagg catactagcc tgatcgggtt cagtccagga
      541 atcctaggtg cgtttatgtc aacctgaatc gaattcccgc ggccgccatg gcggccggga
      601 gcatgcgacg tcgggcccaa ttcgccctat agtgagtcgt attacaattc actggccgtc
      661 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
      721 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
      781 cagttgcgca gcctgaatgg cgaatggacg cgccctgtag cggcgcatta agcgcggcgg
      841 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
      901 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
      961 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     1021 attagg
//
