LOCUS       sequence-166199-        1014 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Use to synthesize riboprobes for in situ hybridization (ISH) probe 
            for Six4. Not for gene expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Parrilla M, Chang I, Degl'Innocenti A, Omura M
  TITLE     Expression of homeobox genes in the mouse olfactory epithelium.
  JOURNAL   J Comp Neurol. 2016 Oct 1;524(14):2713-39. doi: 10.1002/cne.24051. 
            Epub 2016 Jun 19.
  PUBMED    27243442
REFERENCE   2  (bases 1 to 1014)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/cne.24051";
            journalName: "J Comp Neurol"; date: "2016-10-1- 1"; volume: "524"; 
            issue: "14"; pages: "2713-39"
COMMENT     Sequence Label: pISH-Six4
FEATURES             Location/Qualifiers
     source          1..1014
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      7..16
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      11..20
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(728..746)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(729..746)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(764..780)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(764..780)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(777..799)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    788..804
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(812..818,819..836,837..842))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    857..878
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(995..1012)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ctcccggccg ccatggcggc cgcgggaatt cgattccttt atgctgttag tccagggcat
       61 ctctgatttc agaaagaagg cgactttttc taaggtgact tctcagttgc taagcacaaa
      121 gatagtacca ctccttggtt ttgttaaggt ggtggaaggg tttctaacta acacactcat
      181 cagctgagcc tgcagagtaa cggtcactgg ggacttaaca tttgttgtta cacaagggaa
      241 ctttcagtca agattaagag ccctatgaag aattctgtgc ccatcatttt gatcaagaac
      301 ctaaatccca tttttgtgaa attggtgaat gtccctcaat tgctgaaata tgcaatttgg
      361 gccagaggtt gttgtttgtt tgttttggat gttcttgttt tattttcatg aagccagttg
      421 cttcagtgtg gttttccact ctgacactac ttacacagct tggctgcccc cgtctcccag
      481 ttatttactg atcatattaa tgtgattagc atcctggtct tgtagtatag tttacatttt
      541 taaaaaatgt tattttttac tagattgctg cagatatcaa gaggacatta ttttaaaata
      601 cctgtctggt aagggaagtg ggaaagtcaa aacgttctat aaggcggaat cactagtgaa
      661 ttcgcggccg cctgcaggtc gaccatatgg gagagctccc aacgcgttgg atgcatagct
      721 tgagtattct atagtgtcac ctaaatagct tggcgtaatc atggtcatag ctgtttcctg
      781 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
      841 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
      901 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
      961 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgc
//
