LOCUS       sequence-16620-e        1404 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1404)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1995
REFERENCE   2  (bases 1 to 1404)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pPD95_03
FEATURES             Location/Qualifiers
     source          1..1404
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             244..264
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(354..376)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 tgatcttcta actaactatt ctcatttaaa ttttcagagc ttaaaaatgg ctgaaatcac
       61 tcacaacgat ggatacgcta acaacttgga aatgaaataa gcttgcatgc ctgcaggtcg
      121 actctagagg atccccggga ttggccaaag gacccaaagg tatgtttcga atgatactaa
      181 cataacatag aacattttca ggaggaccct tggagggtac cgagctcaga aaaaatgact
      241 gctccaaaga agaagcgtaa ggtaccggtg ggtgaagacc agaaacagca cctcgaactg
      301 agccgcgata ttgcccagcg tttcaacgcg ctgtatggcg agatcgatcc cgtcgtttta
      361 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
      421 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
      481 cgaaggtaag tttaaacaga tccatactaa ctaacttgtt ctgacataat tttcagcttg
      541 aatggcgaat ggcgctttgc ctggtttccg gcaccagaag cggtgccgga aagctggctg
      601 gagtgcgatc ttcctgaggc cgatactgtc gtcgtcccct caaactggca gatgcacggt
      661 tacgatgcgc ccatctacac caacgtgacc tatcccatta cggtcaatcc gccgtttgtt
      721 cccacggaga atccgacggg ttgttactcg ctcacattta atgttgatga aagctggcta
      781 caggaaggcc agacgcgaat tatttttgat ggcgttaact cggcgtttca tctgtggtgc
      841 aacgggcgct gggtcggtta cggccaggta agtttaatta agttgatact aactaacaag
      901 atctgattaa ttttcaggac agtcgtttgc cgtctgaatt tgacctgagc gcatttttac
      961 gcgccggaga aaaccgcctc gcggtgatgg tgctgcnctg gantgacggc agttatctgg
     1021 aagatcagga tatgtggcgg atgancggca ttttccgnga cgtctcgttg ctgcataaac
     1081 cgactacnca aatcagcgat ttccangtgg ccnctcnctt taangatgat tcncccccnc
     1141 tgnactggag gtgaantcng ntagtttnaa cnggatttta ctactanann taacctgant
     1201 ttcnangggg gggggtgggg ganacntcgg gnaanatttn ttttgggggg gaancaagtc
     1261 cccggnnccc ccctttnggg gaanttcaga aggggggttt gcnatccccc ncnctggnnc
     1321 aaacccaang ggggccaanc ccacttnngg gggtatnccc ccnnccttta aaaccntntn
     1381 ttccggggaa aanctcnccn aacc
//
