LOCUS       sequence-166200-        8907 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8907)
  AUTHORS   Greulich H, Kaplan B, Mertins P, Chen TH, Tanaka KE, Yun CH, Zhang 
            X, Lee SH, Cho J, Ambrogio L, Liao R, Imielinski M, Banerji S, 
            Berger AH, Lawrence MS, Zhang J, Pho NH, Walker SR, Winckler W, Getz
            G, Frank D, Hahn WC, Eck MJ, Mani DR, Jaffe JD, Carr SA, Wong KK, 
            Meyerson M
  TITLE     Functional analysis of receptor tyrosine kinase mutations in lung 
            cancer identifies oncogenic extracellular domain mutations of ERBB2.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Sep 4;109(36):14476-81. Epub 2012 Aug
            20.
  PUBMED    22908275
REFERENCE   2  (bases 1 to 8907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2012-09-4"; volume: "109(36)"; pages: 
            "14476-81. Epub 2012 Aug 20"
COMMENT     Sequence Label: pBABEpuro-ERBB2
FEATURES             Location/Qualifiers
     source          1..8907
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             8..477
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    542..741
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             942..1358
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1288..1310
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1326..1342
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     protein_bind    1386..1410
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5176..5200)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(5237..5257)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5242..5571
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5422..5557
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5581..6180
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5581..5600)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6077..6097
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6401..6870
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(7000..7017)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7171..7759)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7251..7270)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7919..8710,8711..8779))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8542..8561
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8780..8884)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgcagcctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca
       61 gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt
      121 tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag
      181 tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc
      241 ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga
      301 gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc
      361 gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc
      421 tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcattt
      481 gggggctcgt ccgggatcgg gagacccctg cccagggacc accgacccac caccgggagg
      541 caagctggcc agcaacttat ctgtgtctgt ccgattgtct agtgtctatg actgatttta
      601 tgcgcctgcg tcggtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa
      661 ctgacgagtt ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc
      721 gtttttgtgg cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg
      781 tggttctggt aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt
      841 cggtttggaa ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt
      901 gtctctgtct gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc
      961 ccttaagttt gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg
     1021 tagatgtcaa gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg
     1081 tcggatggcc gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg
     1141 tcttttcacc tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag
     1201 ccttggcttt tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc
     1261 ctcttcctcc atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat
     1321 cctcccttta tccagccctc actccttctc taggcgccgg ccggatccca gtgtggtggt
     1381 acatcacaag tttgtacaaa aaagttggca tggagctggc ggccttgtgc cgctgggggc
     1441 tcctcctcgc cctcttgccc cccggagccg cgagcaccca agtgtgcacc ggcacagaca
     1501 tgaagctgcg gctccctgcc agtcccgaga cccacctgga catgctccgc cacctctacc
     1561 agggctgcca ggtggtgcag ggaaacctgg aactcaccta cctgcccacc aatgccagcc
     1621 tgtccttcct gcaggatatc caggaggtgc agggctacgt gctcatcgct cacaaccaag
     1681 tgaggcaggt cccactgcag aggctgcgga ttgtgcgagg cacccagctc tttgaggaca
     1741 actatgccct ggccgtgcta gacaatggag acccgctgaa caataccacc cctgtcacag
     1801 gggcctcccc aggaggcctg cgggagctgc agcttcgaag cctcacagag atcttgaaag
     1861 gaggggtctt gatccagcgg aacccccagc tctgctacca ggacacgatt ttgtggaagg
     1921 acatcttcca caagaacaac cagctggctc tcacactgat agacaccaac cgctctcggg
     1981 cctgccaccc ctgttctccg atgtgtaagg gctcccgctg ctggggagag agttctgagg
     2041 attgtcagag cctgacgcgc actgtctgtg ccggtggctg tgcccgctgc aaggggccac
     2101 tgcccactga ctgctgccat gagcagtgtg ctgccggctg cacgggcccc aagcactctg
     2161 actgcctggc ctgcctccac ttcaaccaca gtggcatctg tgagctgcac tgcccagccc
     2221 tggtcaccta caacacagac acgtttgagt ccatgcccaa tcccgagggc cggtatacat
     2281 tcggcgccag ctgtgtgact gcctgtccct acaactacct ttctacggac gtgggatcct
     2341 gcaccctcgt ctgccccctg cacaaccaag aggtgacagc agaggatgga acacagcggt
     2401 gtgagaagtg cagcaagccc tgtgcccgag tgtgctatgg tctgggcatg gagcacttgc
     2461 gagaggtgag ggcagttacc agtgccaata tccaggagtt tgctggctgc aagaagatct
     2521 ttgggagcct ggcatttctg ccggagagct ttgatgggga cccagcctcc aacactgccc
     2581 cgctccagcc agagcagctc caagtgtttg agactctgga agagatcaca ggttacctat
     2641 acatctcagc atggccggac agcctgcctg acctcagcgt cttccagaac ctgcaagtaa
     2701 tccggggacg aattctgcac aatggcgcct actcgctgac cctgcaaggg ctgggcatca
     2761 gctggctggg gctgcgctca ctgagggaac tgggcagtgg actggccctc atccaccata
     2821 acacccacct ctgcttcgtg cacacggtgc cctgggacca gctctttcgg aacccgcacc
     2881 aagctctgct ccacactgcc aaccggccag aggacgagtg tgtgggcgag ggcctggcct
     2941 gccaccagct gtgcgcccga gggcactgct ggggtccagg gcccacccag tgtgtcaact
     3001 gcagccagtt ccttcggggc caggagtgcg tggaggaatg ccgagtactg caggggctcc
     3061 ccagggagta tgtgaatgcc aggcactgtt tgccgtgcca ccctgagtgt cagccccaga
     3121 atggctcagt gacctgtttt ggaccggagg ctgaccagtg tgtggcctgt gcccactata
     3181 aggaccctcc cttctgcgtg gcccgctgcc ccagcggtgt gaaacctgac ctctcctaca
     3241 tgcccatctg gaagtttcca gatgaggagg gcgcatgcca gccttgcccc atcaactgca
     3301 cccactcctg tgtggacctg gatgacaagg gctgccccgc cgagcagaga gccagccctc
     3361 tgacgtccat catctctgcg gtggttggca ttctgctggt cgtggtcttg ggggtggtct
     3421 ttgggatcct catcaagcga cggcagcaga agatccggaa gtacacgatg cggagactgc
     3481 tgcaggaaac ggagctggtg gagccgctga cacctagcgg agcgatgccc aaccaggcgc
     3541 agatgcggat cctgaaagag acggagctga ggaaggtgaa ggtgcttgga tctggcgctt
     3601 ttggcacagt ctacaagggc atctggatcc ctgatgggga gaatgtgaaa attccagtgg
     3661 ccatcaaagt gttgagggaa aacacatccc ccaaagccaa caaagaaatc ttagacgaag
     3721 catacgtgat ggctggtgtg ggctccccat atgtctcccg ccttctgggc atctgcctga
     3781 catccacggt gcagctggtg acacagctta tgccctatgg ctgcctctta gaccatgtcc
     3841 gggaaaaccg cggacgcctg ggctcccagg acctgctgaa ctggtgtatg cagattgcca
     3901 aggggatgag ctacctggag gatgtgcggc tcgtacacag ggacttggcc gctcggaacg
     3961 tgctggtcaa gagtcccaac catgtcaaaa ttacagactt cgggctggct cggctgctgg
     4021 acattgacga gacagagtac catgcagatg ggggcaaggt gcccatcaag tggatggcgc
     4081 tggagtccat tctccgccgg cggttcaccc accagagtga tgtgtggagt tatggtgtga
     4141 ctgtgtggga gctgatgact tttggggcca aaccttacga tgggatccca gcccgggaga
     4201 tccctgacct gctggaaaag ggggagcggc tgccccagcc ccccatctgc accattgatg
     4261 tctacatgat catggtcaaa tgttggatga ttgactctga atgtcggcca agattccggg
     4321 agttggtgtc tgaattctcc cgcatggcca gggaccccca gcgctttgtg gtcatccaga
     4381 atgaggactt gggcccagcc agtcccttgg acagcacctt ctaccgctca ctgctggagg
     4441 acgatgacat gggggacctg gtggatgctg aggagtatct ggtaccccag cagggcttct
     4501 tctgtccaga ccctgccccg ggcgctgggg gcatggtcca ccacaggcac cgcagctcat
     4561 ctaccaggag tggcggtggg gacctgacac tagggctgga gccctctgaa gaggaggccc
     4621 ccaggtctcc actggcaccc tccgaagggg ctggctccga tgtatttgat ggtgacctgg
     4681 gaatgggggc agccaagggg ctgcaaagcc tccccacaca tgaccccagc cctctacagc
     4741 ggtacagtga ggaccccaca gtacccctgc cctctgagac tgatggctac gttgcccccc
     4801 tgacctgcag cccccagcct gaatatgtga accagccaga tgttcggccc cagccccctt
     4861 cgccccgaga gggccctctg cctgctgccc gacctgctgg tgccactctg gaaaggccca
     4921 agactctctc cccagggaag aatggggtcg tcaaagacgt ttttgccttt gggggtgccg
     4981 tggagaaccc cgagtacttg acaccccagg gaggagctgc ccctcagccc caccctcctc
     5041 ctgccttcag cccagccttc gacaacctct attactggga ccaggaccca ccagagcggg
     5101 gggctccacc cagcaccttc aaagggacac ctacggcaga gaacccagag tacctgggtc
     5161 tggacgtgcc agtgtgacca actttcttgt acaaagtggt gatgtaggaa ttcgccagca
     5221 cagtggtcga ccctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
     5281 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
     5341 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
     5401 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     5461 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     5521 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagcttacc
     5581 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta
     5641 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac
     5701 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac
     5761 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag
     5821 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt
     5881 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag
     5941 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc
     6001 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg
     6061 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc
     6121 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga
     6181 cgcccgcccc acgacccgca gcgcccgacc gaaaggagcg cacgacccca tgcatcgata
     6241 aaataaaaga ttttatttag tctccagaaa aaggggggaa tgaaagaccc cacctgtagg
     6301 tttggcaagc tagcttaagt aacgccattt tgcaaggcat ggaaaaatac ataactgaga
     6361 atagagaagt tcagatcaag gtcaggaaca gatggaacag ctgaatatgg gccaaacagg
     6421 atatctgtgg taagcagttc ctgccccggc tcagggccaa gaacagatgg aacagctgaa
     6481 tatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcagg gccaagaaca
     6541 gatggtcccc agatgcggtc cagccctcag cagtttctag agaaccatca gatgtttcca
     6601 gggtgcccca aggacctgaa atgaccctgt gccttatttg aactaaccaa tcagttcgct
     6661 tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat aaaagagccc acaacccctc
     6721 actcggggcg ccagtcctcc gattgactga gtcgcccggg tacccgtgta tccaataaac
     6781 cctcttgcag ttgcatccga cttgtggtct cgctgttcct tgggagggtc tcctctgagt
     6841 gattgactac ccgtcagcgg gggtctttca catgcagcat gtatcaaaat taatttggtt
     6901 ttttttctta agtatttaca ttaaatggcc atagttgcat taatgaatcg gccaacgcgc
     6961 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     7021 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     7081 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     7141 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     7201 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     7261 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     7321 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     7381 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     7441 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     7501 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     7561 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     7621 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     7681 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     7741 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     7801 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     7861 gatccttttg cggccgcaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     7921 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     7981 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     8041 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     8101 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     8161 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     8221 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     8281 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     8341 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     8401 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     8461 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     8521 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     8581 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     8641 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     8701 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     8761 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     8821 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     8881 cgcgcacatt tccccgaaaa gtgccac
//
