LOCUS       sequence-166201-        1090 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Use to synthesize riboprobes for in situ hybridization (ISH) probe 
            for Six4. Not for gene expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Parrilla M, Chang I, Degl'Innocenti A, Omura M
  TITLE     Expression of homeobox genes in the mouse olfactory epithelium.
  JOURNAL   J Comp Neurol. 2016 Oct 1;524(14):2713-39. doi: 10.1002/cne.24051. 
            Epub 2016 Jun 19.
  PUBMED    27243442
REFERENCE   2  (bases 1 to 1090)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/cne.24051";
            journalName: "J Comp Neurol"; date: "2016-10-1- 1"; volume: "524"; 
            issue: "14"; pages: "2713-39"
COMMENT     Sequence Label: pISH-Six4
FEATURES             Location/Qualifiers
     source          1..1090
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      692..701
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      696..705
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(735..754)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(736..754)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(761..778)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(761..777)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(770..792)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(1005..1024)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tccaacgcgt tgggagctct cccatatggt cgacctgcag gcggccgcga attcactagt
       61 gattccgcct tatagaacgt tttgactttc ccacttccct taccagacag gtattttaaa
      121 ataatgtcct cttgatatct gcagcaatct agtaaaaaat aacatttttt aaaaatgtaa
      181 actatactac aagaccagga tgctaatcac attaatatga tcagtaaata actgggagac
      241 gggggcagcc aagctgtgta agtagtgtca gagtggaaaa ccacactgaa gcaactggct
      301 tcatgaaaat aaaacaagaa catccaaaac aaacaaacaa caacctctgg cccaaattgc
      361 atatttcagc aattgaggga cattcaccaa tttcacaaaa atgggattta ggttcttgat
      421 caaaatgatg ggcacagaat tcttcatagg gctcttaatc ttgactgaaa gttcccttgt
      481 gtaacaacaa atgttaagtc cccagtgacc gttactctgc aggctcagct gatgagtgtg
      541 ttagttagaa acccttccac caccttaaca aaaccaagga gtggtactat ctttgtgctt
      601 agcaactgag aagtcacctt agaaaaagtc gccttctttc tgaaatcaga gatgccctgg
      661 actaacagca taaaggaatc gaattcccgc ggccgccatg gcggccggga gcatgcgacg
      721 tcgggcccaa ttcgccctat agtgagtcgt attacaattc actggccgtc gttttacaac
      781 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
      841 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
      901 gcctgaatgg cgaatggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
      961 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     1021 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     1081 tttagggttc
//
