LOCUS       sequence-166205-         999 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Use to synthesize riboprobes for in situ hybridization (ISH) probe 
            for Zfhx4. Not for gene expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 999)
  AUTHORS   Parrilla M, Chang I, Degl'Innocenti A, Omura M
  TITLE     Expression of homeobox genes in the mouse olfactory epithelium.
  JOURNAL   J Comp Neurol. 2016 Oct 1;524(14):2713-39. doi: 10.1002/cne.24051. 
            Epub 2016 Jun 19.
  PUBMED    27243442
REFERENCE   2  (bases 1 to 999)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/cne.24051";
            journalName: "J Comp Neurol"; date: "2016-10-1- 1"; volume: "524"; 
            issue: "14"; pages: "2713-39"
COMMENT     Sequence Label: pISH-Zfhx4
FEATURES             Location/Qualifiers
     source          1..999
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      7..16
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      11..20
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(881..899)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(882..899)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(917..933)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(917..933)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(930..952)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    941..957
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(965..971,972..989,990..995))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 ctcccggccg ccatggcggc cgcgggaatt cgatttgctt ctggcctaga tggtaatttc
       61 aatagtgtcc gaatggatat gttcagtgtg taggagtgaa gacaggatcc cgtgcttaaa
      121 aaaaaattta aaaataaaaa atttttaaaa aaagacttta actgcagttc caaagcttct
      181 ctaacccaaa aattacagta ccaaatgatt gactcaggat tgtttttccc atattgatat
      241 gctggcaata taggatggta tgtggtgaag aagactaatg caggtggttg aatgcgcttg
      301 taatatatgc taaaatatgg aaaaggaaaa aaatatctca caagttcttt gggaacttgt
      361 ttcaagccaa aaactctcaa gacagaaaat tgcacctcag ctgggttgat ttcaaaatgc
      421 cagcatggac agtatgggag gacgatccgg atgtgtcaaa gagaacttct cttagtttag
      481 ttaggtgtaa ttcagtagct ttaaattctc aggtcagaac ataacatttc tcatttgtta
      541 aaagcagcaa gaagcctggt aaaactgtga cttttcccca aatgtcaatc tttattagaa
      601 agcattttct aggtgtgttt agtgtgcaaa gaaactttct aacccttacc ggacaccgca
      661 cagcttcctg cactcaagcc gcagggtagt acagttttac cacagaggga ctcttgagca
      721 atggagtcac gtgtctgccc tgtgtattgc agtttgtctt gacacaagct gtttttacac
      781 cagtgtcatc cctttcttgt aatcactagt gaattcgcgg ccgcctgcag gtcgaccata
      841 tgggagagct cccaacgcgt tggatgcata gcttgagtat tctatagtgt cacctaaata
      901 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
      961 cacacaacat acgagccgga agcataaagt gtaaagcct
//
