LOCUS       sequence-16621-e        1341 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1995
REFERENCE   2  (bases 1 to 1341)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pPD95_07
FEATURES             Location/Qualifiers
     source          1..1341
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             236..256
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(346..368)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 tnantattct catttanatt ttcagagctt aaaaatggct gaaatcactc acaacgatgg
       61 atacgctaac aacttggaaa tgaaataagc ttgcatgcct gcaggtcgac tctagaggat
      121 ccccgggatt ggccaaagga cccaaaggta tgtttcgaat gatactaaca taacatagaa
      181 cattttcagg aggacccttg cttggagggt accgagctca gaaaaaatga ctgctccaaa
      241 gaagaagcgt aaggtaccgg tgggtgaaga ccagaaacag cacctcgaac tgagccgcga
      301 tattgcccag cgtttcaacg cgctgtatgg cgagatcgat cccgtcgttt tacaacgtcg
      361 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
      421 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgaaggta
      481 agtttaaaca gatccatact aactaacttg ttctgacata attttcagct tgaatggcga
      541 atggcgcttt gcctggtttc cggcaccaga agcggtgccg gaaagctggc tggagtgcga
      601 tcttcctgag gccgatactg tcgtcgtccc ctcaaactgg cagatgcacg gttacgatgc
      661 gcccatctac accaacgtga cctatcccat tacggtcaat ccgccgtttg ttcccacgga
      721 gaatccgacg ggttgttact cgctcacatt taatgttgat gaaagctggc tacaggaagg
      781 ccagacgcga attatttttg atggcgttaa ctcggcgttt catctgtggt gcaacgggcg
      841 ctgggtcggt tacggccagg taagtttaat taagttgata ctaactaaca agatctgatt
      901 aattttcagg acagtcgttt gncgtctgaa tttgacctga gcgcattttt acgcgccgga
      961 gaaaaccgcc tcncggtgat ggtgctgcnc tggantgacg gcagttatct ggaagatcag
     1021 gatatgtggc ggatgancgg cattttccgn gacgtctcgt tgctgcataa accgactacc
     1081 caaatcagcg atttccnngt tgccnctcnc ttnaagaaga tttccccnnc cctgtactgg
     1141 aaggctgaan tccnggtant ttaaacggna tnttactaac tanntggcta aactngaatt
     1201 tncnaangng nggggggtgg ggnacccctc cggnaanntt ttnttngggg ngggaaaaaa
     1261 aagtnccngg ggnccggccn tttggnggna aatnnaaaaa ngggggnttn ccatcncccc
     1321 nacctnaaac caaacnaang g
//
