LOCUS       sequence-166212-         779 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPRi/a V2 library parental plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Horlbeck MA, Gilbert LA, Villalta JE, Adamson B, Pak RA, Chen Y, 
            Fields AP, Park CY, Corn JE, Kampmann M, Weissman JS
  TITLE     Compact and highly active next-generation libraries for 
            CRISPR-mediated gene repression and activation.
  JOURNAL   Elife. 2016 Sep 23;5. pii: e19760. doi: 10.7554/eLife.19760.
  PUBMED    27661255
REFERENCE   2  (bases 1 to 779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2016-09-23"; volume: "5. pii: e19760. doi"; pages: " 
            10.7554/eLife.19760"
COMMENT     Sequence Label: pCRISPRia-v2
FEATURES             Location/Qualifiers
     source          1..779
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             16..612
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(16..35)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     512..532
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             622..675
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 gtgagctagc ccaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac
       61 gtcccccggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac
      121 accgtcgacc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg
      181 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc
      241 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg
      301 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg
      361 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag
      421 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg
      481 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc
      541 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc
      601 aagcccggtg ccggatcggg agagggcaga ggaagtctgc taacatgcgg tgacgtcgag
      661 gagaatcctg gcccaccggt cgccaccagc gagctgatta aggagaacat gcacatgaag
      721 ctgtacatgg agggcaccgt ggacaaccat cacttcaagt gcacatccga gggcgaagg
//
