LOCUS       sequence-16622-e        1417 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1995
REFERENCE   2  (bases 1 to 1417)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pPD95_10
FEATURES             Location/Qualifiers
     source          1..1417
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             245..265
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(355..377)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 actgatctta ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat
       61 cactcacaac gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcagg
      121 tcgactagag gatccccggg attggccaaa ggacccaaag gtatgtttcg aatgatacta
      181 acataacata gaacattttc aggaggaccc ttggagggta ccgagctcag aaaaaatgac
      241 tgctccaaag aagaagcgta aggtaccggt gggtgaagac cagaaacagc acctcgaact
      301 gagccgcgat attgcccagc gtttcaacgc gctgtatggc gagatcgatc ccgtcgtttt
      361 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
      421 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
      481 gcgaaggtaa gtttaaacag atccatacta actaacttgt tctgacataa ttttcagctt
      541 gaatggcgaa tggcgctttg cctggtttcc ggcaccagaa gcggtgccgg aaagctggct
      601 ggagtgcgat cttcctgagg ccgatactgt cgtcgtcccc tcaaactggc agatgcacgg
      661 ttacgatgcg cccatctaca ccaacgtgac ctatcccatt acggtcaatc cgccgtttgt
      721 tcccacggag aatccgacgg gttgttactc gctcacattt aatgttgatg aaagctggct
      781 acaggaaggc cagacgcgaa ttatttttga tggcgttaac tcggcgtttc atctgtggtg
      841 caacgggcgc tgggtcggtt acggccaggt aagtttaatt aagttgatac taactaacaa
      901 gatctgatta attttcagga cagtcgtttg ncgtctgaat ttgacctgag cgcattttta
      961 cgcgccggag aaaaccgcct cncggtgatg gtgctgcnct ggaatgacgg cagttatctg
     1021 gnaaaatcag gatatgtggg ggaatgagcg gcattttccg tgacgtctcg ttgctgcana
     1081 anccgactac ccaatcagcg atttccaggt gcccctcnct ttaaggaaga tttccccccn
     1141 ctgnactggg aggctgaant ncnggtaatt tnaacggatn tnactaaata antggctaac
     1201 ctngaatttn cnaanggngg ngngtgngng actncnncgg gaaanntttn ttnggnnggg
     1261 naaaccaggt cccccggcnc cccccttngg ggnaattnna aaaggggggn ttgccntcgc
     1321 cccnccnnga ngcnaaacna anngggggcc ancccccttn nnggggntaa nccccccccc
     1381 ttaaaaaccn gtnttttccn gggnananac ctcccca
//
