LOCUS       sequence-16623-e        1305 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1305)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1995
REFERENCE   2  (bases 1 to 1305)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pPD95_11
FEATURES             Location/Qualifiers
     source          1..1305
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             211..231
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(321..343)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 tcanagctta aaaatggctg anntcactca caacgatgga tacgctaaca acttggaaat
       61 gaaataagct tgcatgcctg caggtcgact ctagaggatc cccgggattg gccaaaggac
      121 ccaaaggtat gtttcgaatg atactaacat aacatagaac attttcagga ggacccttgg
      181 agggtaccga gctcagaaaa aatgactgct ccaaagaaga agcgtaaggt accggtgggt
      241 gaagaccaga aacagcacct cgaactgagc cgcgatattg cccagcgttt caacgcgctg
      301 tatggcgaga tcgatcccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
      361 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
      421 cgcaccgatc gcccttccca acagttgcga aggtaagttt aaacagatcc atactaacta
      481 acttgttctg acataatttt cagcttgaat ggcgaatggc gctttgcctg gtttccggca
      541 ccagaagcgg tgccggaaag ctggctggag tgcgatcttc ctgaggccga tactgtcgtc
      601 gtcccctcaa actggcagat gcacggttac gatgcgccca tctacaccaa cgtgacctat
      661 cccattacgg tcaatccgcc gtttgttccc acggagaatc cgacgggttg ttactcgctc
      721 acatttaatg ttgatgaaag ctggctacag gaaggccaga cgcgaattat ttttgatggc
      781 gttaactcgg cgtttcatct gtggtgcaac gggcgctggg tcggttacgg ccaggtaagt
      841 ttaattaagt tgatactaac taacaagatc tgattaattt tcaggacagt cgtttgccgt
      901 ctgattttga cctgagcgca tttttacgcg ccggagaaaa ccgcctcncg gtgatggtgc
      961 tgccctggga atgacgccag ttatctggaa natcaggata tgtggcggan gaacggcatt
     1021 tttccgngac gtcnccgntg gctgcctaaa ccgactacnc aatcagcgat ttccatgttg
     1081 cccctccctt aaggatgatt ccnccncnct gtacggaagg tgaatccngg tantttaaca
     1141 ggatttataa ctaaatgcta aactngaatt ttcaaagggg ggnngtgggg nnncacctnc
     1201 ggnnaaaatt nnttntggga ggggaancaa gntcccgggg nccncccttt ngggggaaat
     1261 ttcnanagng ggggtttncc aacncccnct tttgaaacca aaccc
//
