LOCUS       sequence-166233-        4953 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4953)
  AUTHORS   Brunet A, Bonni A, Zigmond MJ, Lin MZ, Juo P, Hu LS, Anderson MJ, 
            Arden KC, Blenis J, Greenberg ME
  TITLE     Akt promotes cell survival by phosphorylating and inhibiting a 
            Forkhead transcription factor.
  JOURNAL   Cell 1999 Mar 19;96(6):857-68.
  PUBMED    10102273
REFERENCE   2  (bases 1 to 4953)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell"; 
            date: "1999-03-19"; volume: "96(6)"; pages: "857-68"
COMMENT     Sequence Label: HA-FOXO3a TM
FEATURES             Location/Qualifiers
     source          1..4953
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(63..80)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(234..822)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(314..333)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(993..1784,1785..1853))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1616..1635
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1854..1958)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(2013..2033)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             2348..2374
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     primer_bind     complement(4408..4424)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4408..4424)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     polyA_signal    4539..4673
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4576..4595)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4630..4649
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4732..4751)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct
       61 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
      121 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
      181 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
      241 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
      301 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
      361 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
      421 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
      481 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
      541 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
      601 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
      661 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
      721 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
      781 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
      841 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
      901 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
      961 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     1021 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     1081 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     1141 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     1201 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     1261 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctgc aggcatcgtg
     1321 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     1381 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     1441 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     1501 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     1561 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac acgggataat
     1621 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     1681 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     1741 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     1801 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     1861 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     1921 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     1981 cctgaccttc tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa
     2041 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaag
     2101 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     2161 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     2221 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     2281 cctctgagct attccagaag tagtgaagag gcttttttgg aggagatcta agcttgccgc
     2341 caccatgtat gatgttcctg attatgctag cctcccgggg aattcggcag aggcaccggc
     2401 ttccccggcc ccgctctctc cgctcgaagt ggagctggac ccggagttcg agccccagag
     2461 ccgtccgcga tcctgtgcgt ggcccctgca aaggccggag ctccaagcga gccctgccaa
     2521 gccctcgggg gagacggccg ctgactccat gatccccgag gaggaggacg atgaagacga
     2581 cgaggacggc gggggacggg ccggctcggc catggcgatc ggcggcggcg gcgggagcgg
     2641 cacgctgggc tccgggctgc tccttgagga ctcggcccgg gtgctggcac ccggagggca
     2701 agaccccggg tctgggccag ccaccgcggc gggcgggctg agcgggggta cacaggcgct
     2761 gctgcagcct cagcaaccgc tgccaccgcc gcagccgggg gcggctgggg gctccgggca
     2821 gccgaggaaa tgttcgtcgc ggcggaacgc ctggggaaac ctgtcctacg cggacctgat
     2881 cacccgcgcc atcgagagct ccccggacaa acggctcact ctgtcccaga tctacgagtg
     2941 gatggtgcgt tgcgtgccct acttcaagga taagggcgac agcaacagct ctgccggctg
     3001 gaagaactcc atccggcaca acctgtcact gcatagtcga ttcatgcggg tccagaatga
     3061 gggaactggc aagagctctt ggtggatcat caaccctgat ggggggaaga gcggaaaagc
     3121 cccccggcgg cgggctgtcg caatggacaa tagcaacaag tataccaaga gccgtggccg
     3181 cgcagccaag aagaaggcag ccctgcagac agcccccgaa tcagctgacg acagtccctc
     3241 ccagctctcc aagtggcctg gcagccccac gtcacgcagc agtgatgagc tggatgcgtg
     3301 gacggacttc cgttcacgca ccaatgctaa cgccagcaca gtcagtggcc gcctgtcgcc
     3361 catcatggca agcacagagt tggatgaagt ccaggacgat gatgcgcctc tctcgcccat
     3421 gctctacagc agctcagcca gcctgtcacc ttcagtaagc aagccgtgca cggtggaact
     3481 gccacggctg actgatatgg caggcaccat gaatctgaat gatgggctga ctgaaaacct
     3541 catggacgac ctgctggata acatcacgct cccgccatcc cagccatcgc ccactggggg
     3601 actcatgcag cggagctcta gcttcccgta taccaccaag ggctcgggcc tgggctcccc
     3661 aaccagctcc tttaacagca cggtgttcgg accttcatct ctgaactccc tacgccagtc
     3721 tcccatgcag accatccaag agaacaagcc agctaccttc tcttccatgt cacactatgg
     3781 taaccagaca ctccaggacc tgctcacttc ggactcactt agccacagcg atgtcatgat
     3841 gacacagtcg gaccccttga tgtctcaggc cagcaccgct gtgtctgccc agaattcccg
     3901 ccggaacgtg atgcttcgca atgatccgat gatgtccttt gctgcccagc ctaaccaggg
     3961 aagtttggtc aatcagaact tgctccacca ccagcaccaa acccagggcg ctcttggtgg
     4021 cagccgtgcc ttgtcgaatt ctgtcagcaa catgggcttg agtgagtcca gcagccttgg
     4081 gtcagccaaa caccagcagc agtctcctgt cagccagtct atgcaaaccc tctcggactc
     4141 tctctcaggc tcctccttgt actcaactag tgcaaacctg cccgtcatgg gccatgagaa
     4201 gttccccagc gacttggacc tggacatgtt caatgggagc ttggaatgtg acatggagtc
     4261 cattatccgt agtgaactca tggatgctga tgggttggat tttaactttg attccctcat
     4321 ctccacacag aatgttgttg gtttgaacgt ggggaacttc actggtgcta agcaggcctc
     4381 atctcagagc tgggtgccag gctgaaggat ccactagttc tagataagta atgatcataa
     4441 tcagccatat cacatctgta gaggttttac ttgctttaaa aaacctccca cacctccccc
     4501 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata
     4561 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     4621 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcctctacg
     4681 ccggacgcat cgtggccggc atcaccggcg ccacaggtgc ggttgctggc gcctatatcg
     4741 ccgacatcac cgatggggaa gatcgggctc gccacttcgg gctcatgagc gcttgtttcg
     4801 gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt gggcgccatc tccttgcatg
     4861 caccattcct tgcggcggcg gtgctcaacg gcctcaacct actactgggc tgcttcctaa
     4921 tgcaggagtc gcataaggga gagcgtcgat atg
//
