LOCUS       sequence-166238-        4964 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4964)
  AUTHORS   Brunet A, Sweeney LB, Sturgill JF, Chua KF, Greer PL, Lin Y, Tran H,
            Ross SE, Mostoslavsky R, Cohen HY, Hu LS, Cheng HL, Jedrychowski MP,
            Gygi SP, Sinclair DA, Alt FW, Greenberg ME
  TITLE     Stress-dependent regulation of FOXO transcription factors by the 
            SIRT1 deacetylase.
  JOURNAL   Science 2004 Mar 26;303(5666):2011-5. Epub 2004 Feb 19.
  PUBMED    14976264
REFERENCE   2  (bases 1 to 4964)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science"; 
            date: "2004-03-26"; volume: "303(5666)"; pages: "2011-5. Epub 2004 
            Feb 19"
COMMENT     Sequence Label: FLAG-FOXO3a TM
FEATURES             Location/Qualifiers
     source          1..4964
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(33..53)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             382..405
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(2439..2455)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2439..2455)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     polyA_signal    2570..2704
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2607..2626)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2661..2680
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2763..2782)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(3047..3064)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3218..3806)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3298..3317)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3977..4768,4769..4837))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4600..4619
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4838..4942)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cctgaccttc tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa
       61 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaag
      121 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
      181 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
      241 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
      301 cctctgagct attccagaag tagtgaagag gcttttttgg aggagatcta agcttggtac
      361 cgagctcgga tcctcaggat ggactacaag gacgatgacg ataaacccgg gaattcggca
      421 gaggcaccgg cttccccggc cccgctctct ccgctcgaag tggagctgga cccggagttc
      481 gagccccaga gccgtccgcg atcctgtgcg tggcccctgc aaaggccgga gctccaagcg
      541 agccctgcca agccctcggg ggagacggcc gctgactcca tgatccccga ggaggaggac
      601 gatgaagacg acgaggacgg cgggggacgg gccggctcgg ccatggcgat cggcggcggc
      661 ggcgggagcg gcacgctggg ctccgggctg ctccttgagg actcggcccg ggtgctggca
      721 cccggagggc aagaccccgg gtctgggcca gccaccgcgg cgggcgggct gagcgggggt
      781 acacaggcgc tgctgcagcc tcagcaaccg ctgccaccgc cgcagccggg ggcggctggg
      841 ggctccgggc agccgaggaa atgttcgtcg cggcggaacg cctggggaaa cctgtcctac
      901 gcggacctga tcacccgcgc catcgagagc tccccggaca aacggctcac tctgtcccag
      961 atctacgagt ggatggtgcg ttgcgtgccc tacttcaagg ataagggcga cagcaacagc
     1021 tctgccggct ggaagaactc catccggcac aacctgtcac tgcatagtcg attcatgcgg
     1081 gtccagaatg agggaactgg caagagctct tggtggatca tcaaccctga tggggggaag
     1141 agcggaaaag ccccccggcg gcgggctgtc gcaatggaca atagcaacaa gtataccaag
     1201 agccgtggcc gcgcagccaa gaagaaggca gccctgcaga cagcccccga atcagctgac
     1261 gacagtccct cccagctctc caagtggcct ggcagcccca cgtcacgcag cagtgatgag
     1321 ctggatgcgt ggacggactt ccgttcacgc accaatgcta acgccagcac agtcagtggc
     1381 cgcctgtcgc ccatcatggc aagcacagag ttggatgaag tccaggacga tgatgcgcct
     1441 ctctcgccca tgctctacag cagctcagcc agcctgtcac cttcagtaag caagccgtgc
     1501 acggtggaac tgccacggct gactgatatg gcaggcacca tgaatctgaa tgatgggctg
     1561 actgaaaacc tcatggacga cctgctggat aacatcacgc tcccgccatc ccagccatcg
     1621 cccactgggg gactcatgca gcggagctct agcttcccgt ataccaccaa gggctcgggc
     1681 ctgggctccc caaccagctc ctttaacagc acggtgttcg gaccttcatc tctgaactcc
     1741 ctacgccagt ctcccatgca gaccatccaa gagaacaagc cagctacctt ctcttccatg
     1801 tcacactatg gtaaccagac actccaggac ctgctcactt cggactcact tagccacagc
     1861 gatgtcatga tgacacagtc ggaccccttg atgtctcagg ccagcaccgc tgtgtctgcc
     1921 cagaattccc gccggaacgt gatgcttcgc aatgatccga tgatgtcctt tgctgcccag
     1981 cctaaccagg gaagtttggt caatcagaac ttgctccacc accagcacca aacccagggc
     2041 gctcttggtg gcagccgtgc cttgtcgaat tctgtcagca acatgggctt gagtgagtcc
     2101 agcagccttg ggtcagccaa acaccagcag cagtctcctg tcagccagtc tatgcaaacc
     2161 ctctcggact ctctctcagg ctcctccttg tactcaacta gtgcaaacct gcccgtcatg
     2221 ggccatgaga agttccccag cgacttggac ctggacatgt tcaatgggag cttggaatgt
     2281 gacatggagt ccattatccg tagtgaactc atggatgctg atgggttgga ttttaacttt
     2341 gattccctca tctccacaca gaatgttgtt ggtttgaacg tggggaactt cactggtgct
     2401 aagcaggcct catctcagag ctgggtgcca ggctgaagga tccactagtt ctagataagt
     2461 aatgatcata atcagccata tcacatctgt agaggtttta cttgctttaa aaaacctccc
     2521 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
     2581 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     2641 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     2701 gatcctctac gccggacgca tcgtggccgg catcaccggc gccacaggtg cggttgctgg
     2761 cgcctatatc gccgacatca ccgatgggga agatcgggct cgccacttcg ggctcatgag
     2821 cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc gggggactgt tgggcgccat
     2881 ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc tactactggg
     2941 ctgcttccta atgcaggagt cgcataaggg agagcgtcga tatgcggtgt gaaataccgc
     3001 acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact
     3061 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     3121 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     3181 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     3241 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     3301 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     3361 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     3421 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     3481 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     3541 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     3601 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     3661 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     3721 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     3781 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     3841 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     3901 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     3961 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     4021 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     4081 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     4141 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     4201 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     4261 ttaatagttt gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt
     4321 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     4381 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     4441 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     4501 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     4561 tgcggcgacc gagttgctct tgcccggcgt caacacggga taataccgcg ccacatagca
     4621 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     4681 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     4741 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     4801 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     4861 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     4921 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gcca
//
