LOCUS       sequence-166246-         846 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Sun Q, Westphal W, Wong KN, Tan I, Zhong Q
  TITLE     Rubicon controls endosome maturation as a Rab7 effector.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Nov 9. 107(45):19338-43.
  PUBMED    20974968
REFERENCE   2  (bases 1 to 846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2010 Nov 9. 107(45):19338-43."
COMMENT     Sequence Label: EGFP-Rab7A Q67L
FEATURES             Location/Qualifiers
     source          1..846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             26..52
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             53..79
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        complement(738..756)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(739..756)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(776..793)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 ggacgagctg tacaagggat ccatgtaccc atacgatgtt ccggattacg cttacccata
       61 cgatgttccg gattacgctg gtacctctag gaagaaagtg ttgctgaagg ttatcatcct
      121 gggagattct ggagtcggga agacatcact catgaaccag tatgtgaata agaaattcag
      181 caatcagtac aaagccacaa taggagctga ctttctgacc aaggaggtga tggtggatga
      241 caggctagtc acaatgcaga tatgggacac agcaggactg gaacggttcc agtctctcgg
      301 tgtggccttc tacagaggtg cagactgctg cgttctggta tttgatgtga ctgcccccaa
      361 cacattcaaa accctagata gctggagaga tgagtttctc atccaggcca gtccccgaga
      421 tcctgaaaac ttcccatttg ttgtgttggg aaacaagatt gacctcgaaa acagacaagt
      481 ggccacaaag cgggcacagg cctggtgcta cagcaaaaac aacattccct actttgagac
      541 cagtgccaag gaggccatca acgtggagca ggcgttccag acgattgcac ggaatgcact
      601 taagcaggaa acggaggtgg agctgtacaa cgaatttcct gaacctatca aactggacaa
      661 gaatgaccgg gccaaggcct cggcagaaag ctgcagttgc taagcggccg ctcgagcatg
      721 catctagagg gccctattct atagtgtcac ctaaatgcta gagctcgctg atcagcctcg
      781 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
      841 ctggaa
//
