LOCUS       sequence-16625-e        1302 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1302)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1995
REFERENCE   2  (bases 1 to 1302)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pPD95_18
FEATURES             Location/Qualifiers
     source          1..1302
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             607..627
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(717..739)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 antcncattt anattttcag agcttaaaaa tggctgaaat cactcacaac gatggatacg
       61 ctaacaactt ggaaatgaaa taagcttgca tgcctgcagg ccttggtcga ctctagagga
      121 tccccgggga ttggccaaag gacccaaagg tatgtttcga atgatactaa cataacatag
      181 aacattttca ggaggaccct tgcttggcta gcaaaaatgc ataaggtttt gctggcactg
      241 ttctttatct ttctggcacc agcatccgca ctggcagtct ccgaaccggc ctgcaggtcg
      301 attttttgca aattacgagc gttgtagggg gcggagcgat aggtcctata ggttttggta
      361 tatcatcatt cattcattca ttggtacatt catttaccca ccttcctctt tctgagcttc
      421 tctggagttc tgtgcttcct ttttccctta tctttatact gtaattttta actttcaggc
      481 attgattgga tccccgggat tggccaaagg acccaaaggt atgtttcgaa tgatactaac
      541 ataacataga acattttcag gaggaccctt gcttggaggg taccgagctc agaaaaaatg
      601 actgctccaa agaagaagcg taaggtaccg gtgggtgaag accagaaaca gcacctcgaa
      661 ctgagccgcg atattgccca gcgtttcaac gcgctgtatg gcgagatcga tcccgtcgtt
      721 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
      781 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
      841 ttgcgaaggt aagtttaaac agatccatac taactaactt gttctgacat aattttcagc
      901 ttgaatggcg aatggcgctt tgcctggttt ccggcaccag aagcggtgcc ggaaagctgg
      961 ctggagtgcg atcttcctga ngccganact ggncgtcntc ccctcaaact ggcanangca
     1021 cggttacnan gcgcccatct acnccaacgn gacntatccc attacggnca atccnccgtt
     1081 ggttcccnng naaaanccaa cgggtgntac ncncccccat ttaaanggtg aanaaaactg
     1141 gctacnggaa gnccnanccn aaattttttt gaggggttaa cccggntttt ccttgggggg
     1201 nacngggcct ggggncgntn ccgcccnnaa atttanttaa ntggaaccac caaaaaaccg
     1261 gatattttcg gnaanccctt nncccnnaaa ttaaccaaac na
//
