LOCUS       sequence-166256-         661 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive Expression plasmid with mCherry fluorescent protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 661)
  AUTHORS   Gebhardt MJ, Jacobson RK, Shuman HA
  TITLE     Seeing red; the development of pON.mCherry, a broad-host range 
            constitutive expression plasmid for Gram-negative bacteria.
  JOURNAL   PLoS One. 2017 Mar 3;12(3):e0173116. doi: 
            10.1371/journal.pone.0173116. eCollection 2017.
  PUBMED    28257493
REFERENCE   2  (bases 1 to 661)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2017-03-3"; volume: "12(3):e0173116. doi"; pages: " 
            10.1371/journal.pone.0173116. eCollection 2017"
COMMENT     Sequence Label: pON.mCherry
FEATURES             Location/Qualifiers
     source          1..661
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(290..295,296..311,312..318)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
ORIGIN
        1 actctcttcc gggcgctatc atgccatacc gcgaaaggtt ttgcaccatt cgatggtgtc
       61 aacgtaaatg ccgcttcgcc ttcgcgcgcg aattgcaagc tgatccgggc ttatcgactg
      121 cacggtgcac caatgcttct ggcgtcaggc agccatcgga agctgtggta tggctgtgca
      181 ggtcgtaaat cactgcataa ttcgtgtcgc tcaaggcgca ctcccgttct ggataatgtt
      241 ttttgcgccg acatcataac ggttctggca aatattctga aatgagctgt tgacaattaa
      301 tcatcggctc gtataatgtg tggaaaggcg gttcaccgcc gttttcacac aggaaacaga
      361 attctttaag aaggagatat acatatggtt tccaagggcg aggaggataa catggctatc
      421 attaaagagt tcatgcgctt caaagttcac atggagggtt ctgttaacgg tcacgagttc
      481 gagatcgaag gcgaaggcga gggccgtccg tatgaaggca cccagaccgc caaactgaaa
      541 gtgactaaag gcggcccgct gccttttgcg tgggacatcc tgagcccgca atttatgtac
      601 ggttctaaag cgtatgttaa acacccagcg gatatcccgg actatctgaa gctgtctttt
      661 c
//
