LOCUS       sequence-16626-e        1378 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1378)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1995
REFERENCE   2  (bases 1 to 1378)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pPD95_21
FEATURES             Location/Qualifiers
     source          1..1378
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             625..645
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(735..757)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 tgatcttact aactaactat tctcatttaa attttcagag cttaaaaatg gctgaaatca
       61 ctcacaacga tggatacgct aacaacttgg aaatgaaata agcttgcatg cctgcaggcc
      121 ttggtcgact ctagaggatc cccggggatt ggccaaagga cccaaaggta tgtttcgaat
      181 gatactaaca taacatagaa cattttcagg aggacccttg cttggctagc aaaaatgcat
      241 aaggttttgc tggcactgtt ctttatcttt ctggcaccag catccgcact ggcagtctcc
      301 gaaccggcct gcaggtcgat tttttgcaaa ttacgagcgt tgtagggggc ggagcgatag
      361 gtcctatagg ttttggtata tcatcattca ttcattcatt ggtacattca tttacccacc
      421 ttcctctttc tgagcttctc tggagttctg tgcttccttt ttcccttatc tttatactgt
      481 aatttttaac tttcaggcat tgattggatc cccgggattg gccaaaggac ccaaaggtat
      541 gtttcgaatg atactaacat aacatagaac attttcagga ggacccttgc ttggagggta
      601 ccgagctcag aaaaaatgac tgctccaaag aagaagcgta aggtaccggt gggtgaagac
      661 cagaaacagc acctcgaact gagccgcgat attgcccagc gtttcaacgc gctgtatggc
      721 gagatcgatc ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
      781 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
      841 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgctttgc ctggtttccg
      901 gcaccagaag cggtgccgga aagctggctg gagtgcgatc ttcctgaggc cganactgtc
      961 gtcntcccct caaactggca gangcacggt tacgatgcgc ccatctacac caacgtgacc
     1021 tatcccatta cggtcanncc gcngtttgtt ccccngaaaa tccgacgggt ggtacncncn
     1081 ccatttangn tgangaanct gnctacngga aggccaancc naatattttg aggggtaacn
     1141 cggcgttccc tgggggcacg gggctggntc gttangccaa ananccttng ccccgaatta
     1201 ccgaccattt nacnccngaa aaaccccncc gggaaggcnc cctgaagacc cntttttgga
     1261 aanagaangg gggnaanggg ntttcgnncc cttgngnaaa cnnaaaaaaa aattcngggc
     1321 cccntaaaat nccccncgng ggnnaaanaa ggggnnggnn cccggaantt ttggggaa
//
