LOCUS       sequence-166261-       10499 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Rhesus AAVS1 safe harbor gene targeting donor expressing CAG-driven 
            copGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10499)
  TITLE     Rhesus AAVS1-CAG-copGFP
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10499)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Rhesus AAVS1-CAG-copGFP
FEATURES             Location/Qualifiers
     source          1..10499
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(103..122)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             114..923
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(181..200)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      1101..1689
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1590..1609
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1843..1860
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1977..1998
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2013..2018,2019..2036,2037..2043)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2051..2067
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2056..2078
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2075..2091
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2075..2091
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    complement(2808..2841)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    2842..2867
                     /label=SA
                     /note="splice acceptor site"
     CDS             2891..2944
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             2948..3547
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2948..2967)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3444..3464
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    3548..3772
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    complement(3773..3806)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(5050..5083)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     enhancer        5154..5457
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5459..5736
                     /label=chicken beta-actin promoter
     intron          5738..6755
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     6679..6701
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     6763..6782
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             6843..7505
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
     primer_bind     complement(7667..7686)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    7732..7787
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7786..7805)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     protein_bind    8120..8153
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are 
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     primer_bind     complement(10105..10122)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10105..10121)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10114..10136)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(10330..10349)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10449..10471
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 tgcagctctg gcccgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat
       61 catcatgaac aataaaactg tctgcttaca taaacagtaa tacaaggggt gttatgagcc
      121 atattcaacg ggaaacgtcg aggccgcgat taaattccaa catggatgct gatttatatg
      181 ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat cgcttgtatg
      241 ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt gccaatgatg
      301 ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt ccgaccatca
      361 agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc cccggaaaaa
      421 cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt gatgcgctgg
      481 cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc
      541 gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg
      601 attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa atgcataaac
      661 ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt gataacctta
      721 tttttgacga ggggaaatta ataggttgta ttgatgttgg acgagtcgga atcgcagacc
      781 gataccagga tcttgccatc ctatggaact gcctcggtga gttttctcct tcattacaga
      841 aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg cagtttcatt
      901 tgatgctcga tgagtttttc taatcagaat tggttaattg gttgtaacat tattcagatt
      961 gggcttgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     1021 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     1081 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     1141 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     1201 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     1261 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     1321 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     1381 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     1441 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     1501 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     1561 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     1621 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     1681 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     1741 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     1801 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     1861 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     1921 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     1981 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
     2041 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac
     2101 gccgccacct ccttcaggtt ccagcttcct ccaatccctc ttccccttgc tctgtgctgt
     2161 gcgttgctgc ccaaggatgc tctttccaga gcacttcctt ctcggcgctg caccacgtgg
     2221 tgtcctcgga gcggatcctg cccttgtctg ggtcctctcc gggcatctct gctccctacc
     2281 ccaaccccca tgccatcttc actcgctggg ttcccttttc cctctccttc tggggcctgt
     2341 gccatctctc gtttcttagg atggtcttct ccgtgggatg cctctccctt gcgtccccac
     2401 ctccccttct cgtaggcctg cgtcatcacc gtttttcccg acaaccccaa agtccctgtc
     2461 tccctggctt tagccacctc tccgccctct tgctttcttt gcctggacac cccctcctcc
     2521 tgtggatttg ggtcacctct cacttcttta attcgggcag ctcccatacc ccctttacct
     2581 ctctagtctg tgctcgctct tccggcccct gtcacggcat cttccagggg tcccagagct
     2641 cggctggtcc tcttcctcca agccaggccc ccatggccac ttcaggacaa gatcttgtgt
     2701 cccagagccg ggaccacctt atattcccag gaccgattaa tatggctctg gttctgggta
     2761 cttttatctg taccctccac cccaccgtgg ggccactagg gacaggcata acttcgtata
     2821 gcatacatta tacgaagtta tctgacctct tctcttcctc ccacagggcc tcgagagatc
     2881 tggcagcgga gagggcagag gaagtcttct aacatgcggt gacgtggagg agaatcccgg
     2941 ccctaggatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag
     3001 ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga
     3061 tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg
     3121 gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac
     3181 gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt
     3241 gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc
     3301 caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg
     3361 tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc
     3421 cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt
     3481 caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg
     3541 tgcctgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     3601 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     3661 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     3721 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggataacttc
     3781 gtatagcata cattatacga agttatgagc tcacggggac agcccccccc caaagccccc
     3841 agggatgtaa ttacgtccct cccccgctag ggggcagcag cgagccgccc ggggctccgc
     3901 tccggtccgg cgctcccccc gcatccccga gccggcagcg tgcggggaca gcccgggcac
     3961 ggggaaggtg gcacgggatc gctttcctct gaacgcttct cgctgctctt tgagcctgca
     4021 gacacctggg gggatacggg gaaaaagctt taggctgaaa gagagattta gaatgacaga
     4081 atcatagaac ggcctgggtt gcaaaggagc acagtgctca tccagatcca accccctgct
     4141 atgtgcaggg tcatcaacca gcagcccagg ctgcccagag ccacatccag cctggccttg
     4201 aatgcctgca gggatggggc atccacagcc tccttgggca acctgttcag tgcgtcacca
     4261 ccctctgggg gaaaaactgc ctcctcatat ccaacccaaa cctcccctgt ctcagtgtaa
     4321 agccattccc ccttgtccta tcaaggggga gtttgctgtg acattgttgg tctggggtga
     4381 cacatgtttg ccaattcagt gcatcacgga gaggcagatc ttggggataa ggaagtgcag
     4441 gacagcatgg acgtgggaca tgcaggtgtt gagggctctg ggacactctc caagtcacag
     4501 cgttcagaac agccttaagg ataagaagat aggatagaag gacaaagagc aagttaaaac
     4561 ccagcatgga gaggagcaca aaaaggccac agacactgct ggtccctgtg tctgagcctg
     4621 catgtttgat ggtgtctgga tgcaagcaga aggggtggaa gagcttgcct ggagagatac
     4681 agctgggtca gtaggactgg gacaggcagc tggagaattg ccatgtagat gttcatacaa
     4741 tcgtcaaatc atgaaggctg gaaaagccct ccaagatccc caagaccaac cccaacccac
     4801 ccaccgtgcc cactggccat gtccctcagt gccacatccc cacagttctt catcacctcc
     4861 agggacggtg acccccccac ctccgtgggc agctgtgcca ctgcagcacc gctctttgga
     4921 gaaggtaaat cttgctaaat ccagcccgac cctcccctgg cacaacgtaa ggccattatc
     4981 tctcatccaa ctccaggacg gagtcagtga gaatattggc gcgccgaatt ctcgcgacga
     5041 tcggtcgaca taacttcgta taggatactt tatacgaagt tatcaattca ggctagttat
     5101 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
     5161 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
     5221 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
     5281 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
     5341 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
     5401 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtc
     5461 gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc acccccaatt
     5521 ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg gggggggggg
     5581 cgcgcgccag gcggggcggg gcggggcgag gggcggggcg gggcgaggcg gagaggtgcg
     5641 gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag gcggcggcgg
     5701 cggcggccct ataaaaagcg aagcgcgcgg cgggcgggga gtcgctgcga cgctgccttc
     5761 gccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc ccggctctga ctgaccgcgt
     5821 tactcccaca ggtgagcggg cgggacggcc cttctcctcc gggctgtaat tagcgcttgg
     5881 tttaatgacg gcttgtttct tttctgtggc tgcgtgaaag ccttgagggg ctccgggagg
     5941 gccctttgtg cggggggagc ggctcggggg gtgcgtgcgt gtgtgtgtgc gtggggagcg
     6001 ccgcgtgcgg ctccgcgctg cccggcggct gtgagcgctg cgggcgcggc gcggggcttt
     6061 gtgcgctccg cagtgtgcgc gaggggagcg cggccggggg cggtgccccg cggtgcgggg
     6121 ggggctgcga ggggaacaaa ggctgcgtgc ggggtgtgtg cgtggggggg tgagcagggg
     6181 gtgtgggcgc gtcggtcggg ctgcaacccc ccctgcaccc ccctccccga gttgctgagc
     6241 acggcccggc ttcgggtgcg gggctccgta cggggcgtgg cgcggggctc gccgtgccgg
     6301 gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg gccgcctcgg gccggggagg
     6361 gctcggggga ggggcgcggc ggcccccgga gcgccggcgg ctgtcgaggc gcggcgagcc
     6421 gcagccattg ccttttatgg taatcgtgcg agagggcgca gggacttcct ttgtcccaaa
     6481 tctgtgcgga gccgaaatct gggaggcgcc gccgcacccc ctctagcggg cgcggggcga
     6541 agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg ccttcgtgcg tcgccgcgcc
     6601 gccgtcccct tctccctctc cagcctcggg gctgtccgcg gggggacggc tgccttcggg
     6661 ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac cggcggctct agagcctctg
     6721 ctaaccatgt tcatgccttc ttctttttcc tacagctcct gggcaacgtg ctggttattg
     6781 tgctgtctca tcattttggc aaagaattgt gtacaaccat ggagagcgac gagagcggcc
     6841 tgcccgccat ggagatcgag tgccgcatca ccggcaccct gaacggcgtg gagttcgagc
     6901 tggtgggcgg cggagagggc acccccaagc agggccgcat gaccaacaag atgaagagca
     6961 ccaaaggcgc cctgaccttc agcccctacc tgctgagcca cgtgatgggc tacggcttct
     7021 accacttcgg cacctacccc agcggctacg agaacccctt cctgcacgcc atcaacaacg
     7081 gcggctacac caacacccgc atcgagaagt acgaggacgg cggcgtgctg cacgtgagct
     7141 tcagctaccg ctacgaggcc ggccgcgtga tcggcgactt caaggtggtg ggcaccggct
     7201 tccccgagga cagcgtgatc ttcaccgaca agatcatccg cagcaacgcc accgtggagc
     7261 acctgcaccc catgggcgat aacgtgctgg tgggcagctt cgcccgcacc ttcagcctgc
     7321 gcgacggcgg ctactacagc ttcgtggtgg acagccacat gcacttcaag agcgccatcc
     7381 accccagcat cctgcagaac gggggcccca tgttcgcctt ccgccgcgtg gaggagctgc
     7441 acagcaacac cgagctgggc atcgtggagt accagcacgc cttcaagacc cccatcgcct
     7501 tcgccagatc ccgcgctcag tcgtccaatt ctgccgtgga cggcaccgcc ggacccggct
     7561 ccaccggatc tcgctaaacg cgtgaattca ctcctcaggt gcaggctgcc tatcagaagg
     7621 tggtggctgg tgtggccaat gccctggctc acaaatacca ctgagatctt tttccctctg
     7681 ccaaaaatta tggggacatc atgaagcccc ttgagcatct gacttctggc taataaagga
     7741 aatttatttt cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat
     7801 atgggagggc aaatcattta aaacatcaga atgagtattt ggtttagagt ttggcaacat
     7861 atgcccatat gctggctgcc atgaacaaag gttggctata aagaggtcat cagtatatga
     7921 aacagccccc tgctgtccat tccttattcc atagaaaagc cttgacttga ggttagattt
     7981 tttttatatt ttgttttgtg ttattttttt ctttaacatc cctaaaattt tccttacatg
     8041 ttttactagc cagatttttc ctcctctcct gactactccc agtcatagct gtccctcttc
     8101 tcttatggag atcaccggta taacttcgta taatgtatac tatacgaagt tatggtacct
     8161 taattaagtt aacatgcata gtactgagct cacggggaca gccccccccc aaagccccca
     8221 gggatgtaat tacgtccctc ccccgctagg gggcagcagc gagccgcccg gggctccgct
     8281 ccggtccggc gctccccccg catccccgag ccggcagcgt gcggggacag cccgggcacg
     8341 gggaaggtgg cacgggatcg ctttcctctg aacgcttctc gctgctcttt gagcctgcag
     8401 acacctgggg ggatacgggg aaaaagcttt aggctgaaag agagatttag aatgacagaa
     8461 tcatagaacg gcctgggttg caaaggagca cagtgctcat ccagatccaa ccccctgcta
     8521 tgtgcagggt catcaaccag cagcccaggc tgcccagagc cacatccagc ctggccttga
     8581 atgcctgcag ggatggggca tccacagcct ccttgggcaa cctgttcagt gcgtcaccac
     8641 cctctggggg aaaaactgcc tcctcatatc caacccaaac ctcccctgtc tcagtgtaaa
     8701 gccattcccc cttgtcctat caagggggag tttgctgtga cattgttggt ctggggtgac
     8761 acatgtttgc caattcagtg catcacggag aggcagatct tggggataag gaagtgcagg
     8821 acagcatgga cgtgggacat gcaggtgttg agggctctgg gacactctcc aagtcacagc
     8881 gttcagaaca gccttaagga taagaagata ggatagaagg acaaagagca agttaaaacc
     8941 cagcatggag aggagcacaa aaaggccaca gacactgctg gtccctgtgt ctgagcctgc
     9001 atgtttgatg gtgtctggat gcaagcagaa ggggtggaag agcttgcctg gagagataca
     9061 gctgggtcag taggactggg acaggcagct ggagaattgc catgtagatg ttcatacaat
     9121 cgtcaaatca tgaaggctgg aaaagccctc caagatcccc aagaccaacc ccaacccacc
     9181 caccgtgccc actggccatg tccctcagtg ccacatcccc acagttcttc atcacctcca
     9241 gggacggtga cccccccacc tccgtgggca gctgtgccac tgcagcaccg ctctttggag
     9301 aaggtaaatc ttgctaaatc cagcccgacc ctcccctggc acaacgtaag gccattatct
     9361 ctcatccaac tccaggacgg agtcagtgag aatattgttt gactggtgac aaaaagcccc
     9421 atccttaggc ctcctccctc ctagtctcca gatattgggc ctgaccccca cctcctttta
     9481 agcacattcc ttatctggtg acacaccccc atttcctgga gccacctctc tctccccgcc
     9541 agaaccccta gggtttgctt actgatggag ccagagagga tcctgggagg gagagcttgg
     9601 cggggggtgg gaggtaaggg ggatgcgcga cctgctggtt ctcagtggcc gccctgcgct
     9661 gccctctccc ggagcctgag ctgctctggc gcggccgtct ggtgcatttc actgagccgg
     9721 gtgctgctgc ttccttacgc ttcccaagag gagaagcagt ttggaaaaac aaaatcagaa
     9781 taagttggtc ctgagttcta attttgggtc ttcacctttc tagccccgaa tttgtactgt
     9841 tcctctgtgc atcagttttc cctgtgagat aaggccagta gccgccccgc cctggcaggg
     9901 ctgtggcgag gagggcgtgt ccgtgtggaa aactcccttt gtgagaatgg ttcgtcctag
     9961 gtgttcacca ggtcgtggcc gcctctaccc ccttctcttt ctccatcctt ctttccttaa
    10021 agaggcccag tgctatctgg gacatactcc tccgcccgga gcggggtccc ggggcccctc
    10081 ttccctaagg ccctgctccg aaacactggc cgtcgtttta caacgtcgtg actgggaaaa
    10141 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
    10201 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
    10261 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg
    10321 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
    10381 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
    10441 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcga
//
