LOCUS       sequence-166268-        3837 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing OR2M2, part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3837)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 3837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_OR2M2_p.P182S
FEATURES             Location/Qualifiers
     source          1..3837
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     66..83
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(242..269)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(361..447)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     496..518
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     510..527
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     511..527
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    543..642
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1690..1789)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1806..1825)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1807..1825)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1830..1846)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1830..1846)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2277..3068
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3161..3749
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3650..3669
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
       61 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
      121 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
      181 gcagtgagcg caacgcaatt aatacgcgta ccgctagcca ggaagagttt gtagaaacgc
      241 aaaaaggcca tccgtcagga tggccttctg cttagtttga tgcctggcag tttatggcgg
      301 gcgtcctgcc cgccaccctc cgggccgttg cttcacaacg ttcaaatccg ctcccggcgg
      361 atttgtccta ctcaggagag cgttcaccga caaacaacag ataaaacgaa aggcccagtc
      421 ttccgactga gcctttcgtt ttatttgatg cctggcagtt ccctactctc gcgttaacgc
      481 tagcatggat gttttcccag tcacgacgtt gtaaaacgac ggccagtctt aagctcgggc
      541 cccaaataat gattttattt tgactgatag tgacctgttc gttgcaacaa attgatgagc
      601 aatgcttttt tataatgcca actttgtaca aaaaagttgg caccatggca tgggagaatc
      661 agaccttcaa ctccgacttc atcctccttg gaatcttcaa tcacagccca ccacacacgt
      721 tcctcttctt tctggtcctg ggcatctttt tagtggcctt catgggaaac tctgtcatgg
      781 ttctcctcat ctacctggac acccagctcc acacccccat gtacttcctc ctcagccaac
      841 tgtccctcat ggacctcatg ctcatctgca ccaccgtacc caagatggcc ttcaactact
      901 tgtctggcag caagtccatt tctatggctg gttgtgtcac acaaattttc ttctatatat
      961 cactgtctgg ctctgaatgt tttcttttgg ctgttatggc ttatgaccgc tatattgcta
     1021 tttgccaccc tctaagatat accaatctca tgaatcctaa aatttgtgga cttatggcta
     1081 ccttctcctg gatcctgggc tctacagatg gaatcattga tgctgtagcc acattttcct
     1141 tctccttttg tgggtctcgg gaaatagccc acttcttctg tgaattctct tccctactaa
     1201 tcctctcatg caatgacaca tcaatatttg aagaggttat tttcatctgc tgtatagtaa
     1261 tgcttgtttt ccctgttgca atcatcattg cttcctatgc tggagttatt ctggctgtca
     1321 ttcacatggg atctggagag ggtcgtcgca aagctttcac gacctgttcc tctcacctca
     1381 tggtggtggg aatgtactat ggagcagctt tgttcatgta catacggccc acatctgatc
     1441 actccccaac gcaggacaag atggtgtctg tattctacac catcctcact cccatgctga
     1501 atcccctcat ctacagcctc cgcaacaagg aggtgactag agcattcatg aagatcttag
     1561 gaaagggcaa gtctgagagt gagttacctc ataaacttta tgttttgctg tttgctaaat
     1621 tcttctttct aatatccatc tttttctatg atgtcaaaat actagcattg attatgtaca
     1681 ttgcctaatt gccaactttc ttgtacaaag ttggcattat aagaaagcat tgcttatcaa
     1741 tttgttgcaa cgaacaggtc actatcagtc aaaataaaat cattatttgc catccagctg
     1801 atatccccta tagtgagtcg tattacatgg tcatagctgt ttcctggcag ctctggcccg
     1861 tgtctcaaaa tctctgatgt tacattgcac aagataaaaa tatatcatca tgcctcctct
     1921 agaccagcca ggacagaaat gcctcgactt cgctgctacc caaggttgcc gggtgacgca
     1981 caccgtggaa acggatgaag gcacgaaccc agtggacata agcctgttcg gttcgtaagc
     2041 tgtaatgcaa gtagcgtatg cgctcacgca actggtccag aaccttgacc gaacgcagcg
     2101 gtggtaacgg cgcagtggcg gttttcatgg cttgttatga ctgttttttt ggggtacagt
     2161 ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat
     2221 ggagcagcaa cgatgttacg cagcagggca gtcgccctaa aacaaagtta aacattatga
     2281 gggaagcggt gatcgccgaa gtatcgactc aactatcaga ggtagttggc gtcatcgagc
     2341 gccatctcga accgacgttg ctggccgtac atttgtacgg ctccgcagtg gatggcggcc
     2401 tgaagccaca cagtgatatt gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa
     2461 cgcggcgagc tttgatcaac gaccttttgg aaacttcggc ttcccctgga gagagcgaga
     2521 ttctccgcgc tgtagaagtc accattgttg tgcacgacga catcattccg tggcgttatc
     2581 cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa tgacattctt gcaggtatct
     2641 tcgagccagc cacgatcgac attgatctgg ctatcttgct gacaaaagca agagaacata
     2701 gcgttgcctt ggtaggtcca gcggcggagg aactctttga tccggttcct gaacaggatc
     2761 tatttgaggc gctaaatgaa accttaacgc tatggaactc gccgcccgac tgggctggcg
     2821 atgagcgaaa tgtagtgctt acgttgtccc gcatttggta cagcgcagta accggcaaaa
     2881 tcgcgccgaa ggatgtcgct gccgactggg caatggagcg cctgccggcc cagtatcagc
     2941 ccgtcatact tgaagctaga caggcttatc ttggacaaga agaagatcgc ttggcctcgc
     3001 gcgcagatca gttggaagaa tttgtccact acgtgaaagg cgagatcacc aaggtagtcg
     3061 gcaaataacc ctcgagccac ccatgaccaa aatcccttaa cgtgagttac gcgtcgttcc
     3121 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     3181 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     3241 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     3301 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     3361 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     3421 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     3481 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     3541 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     3601 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     3661 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     3721 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     3781 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctg
//
