LOCUS       sequence-166275-        3918 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing SPOP, part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3918)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 3918)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_SPOP_p.F133S
FEATURES             Location/Qualifiers
     source          1..3918
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      25..613
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     514..533
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     767..784
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(943..970)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1062..1148)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1197..1219
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1211..1228
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1212..1228
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1244..1343
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2472..2571)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2588..2607)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2589..2607)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2612..2628)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2612..2628)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3059..3850
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
       61 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
      121 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
      181 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
      241 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
      301 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
      361 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
      421 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
      481 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
      541 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
      601 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
      661 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
      721 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
      781 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
      841 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
      901 taatacgcgt accgctagcc aggaagagtt tgtagaaacg caaaaaggcc atccgtcagg
      961 atggccttct gcttagtttg atgcctggca gtttatggcg ggcgtcctgc ccgccaccct
     1021 ccgggccgtt gcttcacaac gttcaaatcc gctcccggcg gatttgtcct actcaggaga
     1081 gcgttcaccg acaaacaaca gataaaacga aaggcccagt cttccgactg agcctttcgt
     1141 tttatttgat gcctggcagt tccctactct cgcgttaacg ctagcatgga tgttttccca
     1201 gtcacgacgt tgtaaaacga cggccagtct taagctcggg ccccaaataa tgattttatt
     1261 ttgactgata gtgacctgtt cgttgcaaca aattgatgag caatgctttt ttataatgcc
     1321 aactttgtac aaaaaagttg gcaccatgtc aagggttcca agtcctccac ctccggcaga
     1381 aatgtcgagt ggccccgtag ctgagagttg gtgctacaca cagatcaagg tagtgaaatt
     1441 ctcctacatg tggaccatca ataactttag cttttgccgg gaggaaatgg gtgaagtcat
     1501 taaaagttct acattttcat caggagcaaa tgataaactg aaatggtgtt tgcgagtaaa
     1561 ccccaaaggg ttagatgaag aaagcaaaga ttacctgtca ctttacctgt tactggtcag
     1621 ctgtccaaag agtgaagttc gggcaaaatt caaattctcc atcctgaatg ccaagggaga
     1681 agaaaccaaa gctatggaga gtcaacgggc atataggttt gtgcaaggca aagactgggg
     1741 atccaagaaa ttcatccgta gagattttct tttggatgag gccaacgggc ttctccctga
     1801 tgacaagctt accctcttct gcgaggtgag tgttgtgcaa gattctgtca acatttctgg
     1861 ccagaatacc atgaacatgg taaaggttcc tgagtgccgg ctggcagatg agttaggagg
     1921 actgtgggag aattcccggt tcacagactg ctgcttgtgt gttgccggcc aggaattcca
     1981 ggctcacaag gctatcttag cagctcgttc tccggttttt agtgccatgt ttgaacatga
     2041 aatggaggag agcaaaaaga atcgagttga aatcaatgat gtggagcctg aagtttttaa
     2101 ggaaatgatg tgcttcattt acacggggaa ggctccaaac ctcgacaaaa tggctgatga
     2161 tttgctggca gctgctgaca agtatgccct ggagcgctta aaggtcatgt gtgaggatgc
     2221 cctctgcagt aacctgtccg tggagaacgc tgcagaaatt ctcatcctgg ccgacctcca
     2281 cagtgcagat cagttgaaaa ctcaggcagt ggatttcatc aactatcatg cttcggatgt
     2341 cttggagacc tctgggtgga agtcaatggt ggtgtcacat ccccacttgg tggctgaggc
     2401 ataccgctct ctggcttcag cacagtgccc ttttctggga cccccacgca aacgcctgaa
     2461 gcaatcctaa ttgccaactt tcttgtacaa agttggcatt ataagaaagc attgcttatc
     2521 aatttgttgc aacgaacagg tcactatcag tcaaaataaa atcattattt gccatccagc
     2581 tgatatcccc tatagtgagt cgtattacat ggtcatagct gtttcctggc agctctggcc
     2641 cgtgtctcaa aatctctgat gttacattgc acaagataaa aatatatcat catgcctcct
     2701 ctagaccagc caggacagaa atgcctcgac ttcgctgcta cccaaggttg ccgggtgacg
     2761 cacaccgtgg aaacggatga aggcacgaac ccagtggaca taagcctgtt cggttcgtaa
     2821 gctgtaatgc aagtagcgta tgcgctcacg caactggtcc agaaccttga ccgaacgcag
     2881 cggtggtaac ggcgcagtgg cggttttcat ggcttgttat gactgttttt ttggggtaca
     2941 gtctatgcct cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt
     3001 atggagcagc aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt taaacattat
     3061 gagggaagcg gtgatcgccg aagtatcgac tcaactatca gaggtagttg gcgtcatcga
     3121 gcgccatctc gaaccgacgt tgctggccgt acatttgtac ggctccgcag tggatggcgg
     3181 cctgaagcca cacagtgata ttgatttgct ggttacggtg accgtaaggc ttgatgaaac
     3241 aacgcggcga gctttgatca acgacctttt ggaaacttcg gcttcccctg gagagagcga
     3301 gattctccgc gctgtagaag tcaccattgt tgtgcacgac gacatcattc cgtggcgtta
     3361 tccagctaag cgcgaactgc aatttggaga atggcagcgc aatgacattc ttgcaggtat
     3421 cttcgagcca gccacgatcg acattgatct ggctatcttg ctgacaaaag caagagaaca
     3481 tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt gatccggttc ctgaacagga
     3541 tctatttgag gcgctaaatg aaaccttaac gctatggaac tcgccgcccg actgggctgg
     3601 cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa
     3661 aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg cccagtatca
     3721 gcccgtcata cttgaagcta gacaggctta tcttggacaa gaagaagatc gcttggcctc
     3781 gcgcgcagat cagttggaag aatttgtcca ctacgtgaaa ggcgagatca ccaaggtagt
     3841 cggcaaataa ccctcgagcc acccatgacc aaaatccctt aacgtgagtt acgcgtcgtt
     3901 ccactgagcg tcagaccc
//
