LOCUS       sequence-166276-       10228 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive TEF1 promoter mediated expression of the UBiC, ARO4*, 
            and TKL1 genes .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10228)
  AUTHORS   Williams TC, Peng B, Vickers CE, Nielsen LK
  TITLE     The Saccharomyces cerevisiae pheromone-response is a metabolically 
            active stationary phase for bio-production.
  JOURNAL   Metab Eng Commun. 2016 May 11;3:142-152. doi: 
            10.1016/j.meteno.2016.05.001. eCollection 2016 Dec.
  PUBMED    29468120
REFERENCE   2  (bases 1 to 10228)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng 
            Commun."; date: "2016-05-11"; volume: "3:142-152. doi"; pages: " 
            10.1016/j.meteno.2016.05.001. eCollection 2016 Dec"
COMMENT     Sequence Label: PHBA03
FEATURES             Location/Qualifiers
     source          1..10228
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     240..260
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        242..260
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     terminator      complement(311..558)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     promoter        complement(2645..3051)
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     terminator      complement(3099..3346)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     3372..3388
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(4521..4909)
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     terminator      complement(4988..5235)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     5261..5277
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(5795..6201)
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     primer_bind     complement(6243..6262)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6244..6262)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6272..6289)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6272..6288)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6281..6303)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6429..6884
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6516..6535)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6726..6747
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(7015..7818)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(7819..8034)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(8058..8077)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8177..8199
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8237..8255)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8323..8427
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8428..8496,8497..9288)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8646..8665)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9459..10047
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9948..9967
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10201..10218
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg
      241 caattaaccc tcactaaagg gaacaaaagc tggagctcca ccgacgatga gagtgtaaac
      301 tgcgaagctt gcaaattaaa gccttcgagc gtcccaaaac cttctcaagc aaggttttca
      361 gtataatgtt acatgcgtac acgcgtttgt acagaaaaaa aagaaaaatt tgaaatataa
      421 ataacgttct taatactaac ataactataa aaaaataaat agggacctag acttcaggtt
      481 gtctaactcc ttccttttcg gttagagcgg atgtgggggg agggcgtgaa tgtaagcgtg
      541 acataactaa ttacatgata tcgacaaagg aaaaggggcc tgttctagat tagaaagctt
      601 ttttcaaagg agaaattagc ttgtcaccct tatagaatgc aatggtcttt tgagctcttt
      661 cagcaacacc ttctggggtg aaaccgaaga acttgaagac ttctggtgcc ttaccggagg
      721 caccaaatct gtcaataccg aaggattgat gagcgtattt gccccaacat gtggtagcca
      781 aaacttcaac agacatgatt ggaacgttgt ctggtaagac tgatagtctg tattctaggg
      841 gttgtttgtc aaaagtgaag aaatctggta gagaaacaac acgagccttg atgttctttg
      901 cggccaaagt cttagcagct tcaacactca aagacacttc ggaaccagta gccactaaaa
      961 taatatctgg gttagcaaca tcttgtagta cgtaaccacc cttagaagcg ctttcaatag
     1021 agctaccttc caattgtggc aagttttgtc tggacaaagc aatgatactt ggagtatgct
     1081 tggattctaa agagttcttg taggcggcag aaacttcgtt accatcagct ggtctccaaa
     1141 cttgaatgtt tggtagggat ctgaagtgtg ctaaagtttc aataggttga tgtgttggac
     1201 catcttcacc gacaccgata gagtcatgtg tagcaaccca aataactggg tggccagaca
     1261 aagcggacaa tctaacggca ccagcagcat aagaaacgaa gttcaagaaa gtaccaccgt
     1321 atggtttgta gttggcaccg aaagctgaaa taccgttcat tatggcaccc atagcgtgtt
     1381 ctctaatacc gtacctaatg tatctaccag agtagttacc tgaaccggaa gaaggaggtt
     1441 ggaagtcaag ggcttccttc catctggtca agttagaagg tgttaaatcg gcagaaccac
     1501 caatcaactc tggcaattga ttgtaaacat cctcaagaac agtttctgat aattttctag
     1561 tggccacggc agagtccttg gcggtgtaag ttggcaactt agattcccaa tttgcgggta
     1621 gttggccgct caatcttcta gccaattcag cacctaattc tgggaatttc ttttggtatt
     1681 cgctgaacaa cttgttccac ttgttgttgg cttcgacacc tggctttaaa attgtctttt
     1741 ggtagtggtc gtaaacttct tgtggaacaa caaaggactt gtctgggttg aaaccgaatt
     1801 tgctctttag ttgtttaaca tcatctgctt tcaatggggc accgtgcaca gagtgagagc
     1861 cggcatgcaa ggaaccgtaa ccaatggttg tggtcatttt gatcaaagtt ggtttgtcct
     1921 tggataactt agcttgagca atagccttgg caataccggc tagatcttcg ttaccatttt
     1981 ctacgtacaa aacttcccaa ccgtaggctt cgtatctctt agcaacatct tcatcgaatg
     2041 agatactggt agcaccatcg atagtgatct tgttgtcatc gtagatggca atcaagttac
     2101 ccaatttcaa atgaccagcc aaggaggaag cttctgaaga aataccttct tgcaaacaac
     2161 cgtcacccaa gaaaacatag gtgtagttgt cagacaaggt aaagcccggc ttgttgtaag
     2221 tggcagccag gttagcttga gccatggcca taccaacagc gttggagata ccttgaccta
     2281 atggaccggt agtaacttca acacctggca actcaaattc aggatgacct ggtgttctgg
     2341 aacccaactg tctgaactgt ttcaagtctt caatagacag atcgtaacca gtcaaatgta
     2401 gcatagaata caacaaagcg accgcgtgac cgttagacaa gacaaatcta tctctgttga
     2461 tccagtctgg gttggttggg ttcatgcgca tttgactcca tagaacgtgt gcagctggtg
     2521 ccatacccaa tggagcacct gggtgacctg agttggcctt ggatacggtg tccacagcca
     2581 aaattcttat ggtggagacg gctagcttat caatgtcagt gaattgagtc atttgtaatt
     2641 aaaacttaga ttagattgct atgctttctt tctaatgagc aagaagtaaa aaaagttgta
     2701 atagaacaag aaaaatgaaa ctgaaacttg agaaattgaa gaccgtttat taacttaaat
     2761 atcaatggga ggtcatcgaa agagaaaaaa atcaaaaaaa aaaattttca agaaaaagaa
     2821 acgtgataaa aatttttatt gcctttttcg acgaagaaaa agaaacgagg cggtctcttt
     2881 tttcttttcc aaacctttag tacgggtaat taacgacacc ctagaggaag aaagagggga
     2941 aatttagtat gctgtgcttg ggtgttttga agtggtacgg cgatgcgcgg agtccgagaa
     3001 aatctggaag agtaaaaaag gagtagaaac attttgaagc tatggtgtgt gcctcgagcg
     3061 gtggcggccg cacgatgaga gtgtaaactg cgaagcttgc aaattaaagc cttcgagcgt
     3121 cccaaaacct tctcaagcaa ggttttcagt ataatgttac atgcgtacac gcgtttgtac
     3181 agaaaaaaaa gaaaaatttg aaatataaat aacgttctta atactaacat aactataaaa
     3241 aaataaatag ggacctagac ttcaggttgt ctaactcctt ccttttcggt tagagcggat
     3301 gtggggggag ggcgtgaatg taagcgtgac ataactaatt acatgatatc gacaaaggaa
     3361 aaggggcctg ttctagaact agtggatccc ccgggctatt tcttgttaac ttctcttctt
     3421 tgtctgacag cagcagccaa tttcctcaag acgtcttcag tagtttccca acctatacaa
     3481 gcatcagtga tggaaacacc atatttcaag ccggctttac cttcggctgg gatgccttgg
     3541 ttaccttcgt tgatgtttga ttcaatcatg acaccggtaa tggcgttttc accgttagcg
     3601 atttgctcac aaacaacgtc attgaccttt ggttggtttc tgaaatcctt attggagtta
     3661 ccgtgagagt agtcaatcat tagaccgttg gaaccggcag gcaattgagc cttagcttct
     3721 gcaacggact tagcgtcgta gttggtaccc tttttaccac ctcttagaat aacgaagcag
     3781 tgttcgttac ccttagtagt ggtgatagca gcaacaccat gcttagtaac acccatgaaa
     3841 tggtgagaat gagcagcggc ttgacaagca tccacagcaa catttaaggt accatcggta
     3901 ccgttcttga aaccaactgg gaaagacaaa ccggaggcca attctctgtg cagttgagat
     3961 tcggtggttc tggcaccaat ggcaccgaag gagaccaaat cagccaagta tttaggagaa
     4021 atggtatcaa gcatttcaga accaattggc aaaccgatat ttgtcaagtt gacaaacaat
     4081 tgtctagcgg attgcaaacc cttgttgatg ttgaaagtgt tgttaacatc agggtcatta
     4141 attagacctt tccagccgac ggttgttctt ggcttctcca agtatgctct cataatgatg
     4201 gataaatcac cttttaattc atctgacaat ttctttaatc tcaaagcgta ttcttgagcg
     4261 gcttctagat catggatgga acaaggaccg acaatgacaa gaactctgtc gtctttaccg
     4321 gtaataatat ctatagcttc tcttctacct ctcttggcag tttccaaaga agttggtgtg
     4381 gctgggattt gcacttgaag gagagctgga gaagctaatg ggtcgtaacc taaaattctg
     4441 acatcttctt cagcaccttg atttaccttt ggcatgccgt tggcagcgaa cattggagat
     4501 tcactcattt gtaattaaaa cttagattag attgctatgc tttctttcta atgagcaaga
     4561 agtaaaaaaa gttgtaatag aacaagaaaa atgaaactga aacttgagaa attgaagacc
     4621 gtttattaac ttaaatatca atgggaggtc atcgaaagag aaaaaaatca aaaaaaaaaa
     4681 ttttcaagaa aaagaaacgt gataaaaatt tttattgcct ttttcgacga agaaaaagaa
     4741 acgaggcggt ctcttttttc ttttccaaac ctttagtacg ggtaattaac gacaccctag
     4801 aggaagaaag aggggaaatt tagtatgctg tgcttgggtg ttttgaagtg gtacggcgat
     4861 gcgcggagtc cgagaaaatc tggaagagta aaaaaggagt agaaacattt tgaagcacga
     4921 tgagagtgta aactgctatg gtgtgtgcct cgaggaattc acgatgagag tgtaaactgc
     4981 gaagcttgca aattaaagcc ttcgagcgtc ccaaaacctt ctcaagcaag gttttcagta
     5041 taatgttaca tgcgtacacg cgtttgtaca gaaaaaaaag aaaaatttga aatataaata
     5101 acgttcttaa tactaacata actataaaaa aataaatagg gacctagact tcaggttgtc
     5161 taactccttc cttttcggtt agagcggatg tggggggagg gcgtgaatgt aagcgtgaca
     5221 taactaatta catgatatcg acaaaggaaa aggggcctgt tctagaacta gtggatcccc
     5281 cgggtcagta taatggggaa gctggcaaga ataattcggt caacaacaaa ggtttaccag
     5341 acaatctcaa tcttgatctt ctaccccaca aaccagcatc tctaccaatt tcaatgaagt
     5401 ctctggtcaa ggtagaagaa gtgaacaagt atctacccaa tggagtttta cccaattttt
     5461 gcaatgccaa ttctggacca gacaaagtag aaactggaac aacagttcta ccagccaacc
     5521 aaggttcacc atcagcgcac aacaaaattt ctctcaacca gtatctagat tcttttggca
     5581 acaaaggcaa ttcttctggg atttcgtttt gttcgacgaa accttctcta atcatggtaa
     5641 cggaaacggt cttaccttgt tgttcgaatc tcttagtcat ggaatcttcc aacaacaacc
     5701 agtccaataa ttgtggatcc aatgctggaa tttctttgca gtatctcaaa gctctcaatt
     5761 gtgtcaaagc tggatgggac atttgtaatt aaaacttaga ttagattgct atgctttctt
     5821 tctaatgagc aagaagtaaa aaaagttgta atagaacaag aaaaatgaaa ctgaaacttg
     5881 agaaattgaa gaccgtttat taacttaaat atcaatggga ggtcatcgaa agagaaaaaa
     5941 atcaaaaaaa aaaattttca agaaaaagaa acgtgataaa aatttttatt gcctttttcg
     6001 acgaagaaaa agaaacgagg cggtctcttt tttcttttcc aaacctttag tacgggtaat
     6061 taacgacacc ctagaggaag aaagagggga aatttagtat gctgtgcttg ggtgttttga
     6121 agtggtacgg cgatgcgcgg agtccgagaa aatctggaag agtaaaaaag gagtagaaac
     6181 attttgaagc tatggtgtgt gcctcgaggt cgacctcgag ggggggcccg gtacccaatt
     6241 cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact
     6301 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
     6361 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
     6421 gcgaatggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     6481 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     6541 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     6601 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     6661 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     6721 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     6781 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     6841 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttcctgat gcggtatttt
     6901 ctccttacgc atctgtgcgg tatttcacac cgcatagggt aataactgat ataattaaat
     6961 tgaagctcta atttgtgagt ttagtataca tgcatttact tataatacag ttttttagtt
     7021 ttgctggccg catcttctca aatatgcttc ccagcctgct tttctgtaac gttcaccctc
     7081 taccttagca tcccttccct ttgcaaatag tcctcttcca acaataataa tgtcagatcc
     7141 tgtagagacc acatcatcca cggttctata ctgttgaccc aatgcgtctc ccttgtcatc
     7201 taaacccaca ccgggtgtca taatcaacca atcgtaacct tcatctcttc cacccatgtc
     7261 tctttgagca ataaagccga taacaaaatc tttgtcgctc ttcgcaatgt caacagtacc
     7321 cttagtatat tctccagtag atagggagcc cttgcatgac aattctgcta acatcaaaag
     7381 gcctctaggt tcctttgtta cttcttctgc cgcctgcttc aaaccgctaa caatacctgg
     7441 gcccaccaca ccgtgtgcat tcgtaatgtc tgcccattct gctattctgt atacacccgc
     7501 agagtactgc aatttgactg tattaccaat gtcagcaaat tttctgtctt cgaagagtaa
     7561 aaaattgtac ttggcggata atgcctttag cggcttaact gtgccctcca tggaaaaatc
     7621 agtcaagata tccacatgtg tttttagtaa acaaattttg ggacctaatg cttcaactaa
     7681 ctccagtaat tccttggtgg tacgaacatc caatgaagca cacaagtttg tttgcttttc
     7741 gtgcatgata ttaaatagct tggcagcaac aggactagga tgagtagcag cacgttcctt
     7801 atatgtagct ttcgacatga tttatcttcg tttcctgcag gtttttgttc tgtgcagttg
     7861 ggttaagaat actgggcaat ttcatgtttc ttcaacacta catatgcgta tatataccaa
     7921 tctaagtctg tgctccttcc ttcgttcttc cttctgttcg gagattaccg aatcaaaaaa
     7981 atttcaagga aaccgaaatc aaaaaaaaga ataaaaaaaa aatgatgaat tgaaaaggtg
     8041 gtatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac
     8101 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
     8161 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
     8221 cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     8281 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     8341 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     8401 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     8461 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     8521 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     8581 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     8641 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     8701 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     8761 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     8821 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     8881 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     8941 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     9001 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     9061 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     9121 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     9181 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     9241 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     9301 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     9361 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     9421 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     9481 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     9541 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     9601 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     9661 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     9721 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     9781 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     9841 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     9901 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     9961 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
    10021 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
    10081 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
    10141 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
    10201 agcgagtcag tgagcgagga agcggaag
//
