LOCUS       sequence-16628-e        1264 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1264)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1995
REFERENCE   2  (bases 1 to 1264)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pPD95_27
FEATURES             Location/Qualifiers
     source          1..1264
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             634..654
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(744..766)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 gnnnttaaat gatcttacta actaactatt ctcatttaaa ttttcagagc ttaaaaatgg
       61 ctgaaatcac tcacaacgat ggatacgcta acaacttgga aatgaaataa gcttgcatgc
      121 ctgcaggcct tggtcgactc tagaggatcc ccggggattg gccaaaggac ccaaaggtat
      181 gtttcgaatg atactaacat aacatagaac attttcagga ggacccttgc ttggctagca
      241 aaaatgcata aggttttgct ggcactgttc tttatctttc tggcaccagc atccgcactg
      301 gcagtctccg aaccggcctg caggtcgatt ttttgcaaat tacgagcgtt gtagggggcg
      361 gagcgatagg tcctataggt tttggtatat catcattcat tcattcattg gtacattcat
      421 ttacccacct tcctctttct gagcttctct ggagttctgt gcttcctttt tcccttatct
      481 ttatactgta atttttaact ttcaggcatt gattggatcc ccgggattgg ccaaaggacc
      541 caaaggtatg tttcgaatga tactaacata acatagaaca ttttcaggag gacccttgct
      601 tggagggtac cgagctcaga aaaaatgact gctccaaaga agaagcgtaa ggtaccggtg
      661 ggtgaagacc agaaacagca cctcgaactg agccgcgata ttgcccagcg tttcaacgcg
      721 ctgtatggcg agatcgatcc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
      781 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
      841 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgctttgcc
      901 tggtttccgg caccagaagc ggtgccggaa agctggctgg agtgcgatct tcctgangcc
      961 gatactgtcg tcgtcccctc aaactggcag angcacggtt acgatgcgcc catctacacc
     1021 aacgtgacct atcccattac ggtcaatccg cngtttgtnc ccccggaaaa nccgannggn
     1081 tgntacncnc cnccatttaa angttganga aagctggcta cngnaaggcc aaacccnaat
     1141 tantttttga aggggntaac tcnggggttt cntcnggggg ggcancnggg ncctgggtcc
     1201 gttaacgncn aaaaanancc nttngccccc ngaaattnaa cnggaggcat ttttnccccc
     1261 cgga
//
