LOCUS       sequence-166281-        3837 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing OR2M2, part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3837)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 3837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_OR2M2_p.N42Y
FEATURES             Location/Qualifiers
     source          1..3837
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      50..638
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     539..558
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     792..809
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(968..995)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1087..1173)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1222..1244
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1236..1253
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1237..1253
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1269..1368
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2416..2515)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2532..2551)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2533..2551)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2556..2572)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2556..2572)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3003..3794
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
       61 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
      121 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
      181 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
      241 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
      301 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
      361 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
      421 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
      481 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
      541 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
      601 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
      661 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
      721 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
      781 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
      841 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
      901 aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa gagtttgtag
      961 aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc tggcagttta
     1021 tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca aatccgctcc
     1081 cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa aacgaaaggc
     1141 ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct actctcgcgt
     1201 taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc agtcttaagc
     1261 tcgggcccca aataatgatt ttattttgac tgatagtgac ctgttcgttg caacaaattg
     1321 atgagcaatg cttttttata atgacaactt tgtacaaaaa agttggcacc atggcatggg
     1381 agaatcagac cttcaactcc gacttcatcc tccttggaat cttcaatcac agcccaccac
     1441 acacgttcct cttctttctg gtcctgggca tctttttagt ggccttcatg ggatactctg
     1501 tcatggttct cctcatctac ctggacaccc agctccacac ccccatgtac ttcctcctca
     1561 gccaactgtc cctcatggac ctcatgctca tctgcaccac cgtacccaag atggccttca
     1621 actacttgtc tggcagcaag tccatttcta tggctggttg tgtcacacaa attttcttct
     1681 atatatcact gtctggctct gaatgttttc ttttggctgt tatggcttat gaccgctata
     1741 ttgctatttg ccaccctcta agatatacca atctcatgaa tcctaaaatt tgtggactta
     1801 tggctacctt ctcctggatc ctgggctcta cagatggaat cattgatgct gtagccacat
     1861 tttccttctc cttttgtggg tctcgggaaa tagcccactt cttctgtgaa ttcccttccc
     1921 tactaatcct ctcatgcaat gacacatcaa tatttgaaga ggttattttc atctgctgta
     1981 tagtaatgct tgttttccct gttgcaatca tcattgcttc ctatgctgga gttattctgg
     2041 ctgtcattca catgggatct ggagagggtc gtcgcaaagc tttcacgacc tgttcctctc
     2101 acctcatggt ggtgggaatg tactatggag cagctttgtt catgtacata cggcccacat
     2161 ctgatcactc cccaacgcag gacaagatgg tgtctgtatt ctacaccatc ctcactccca
     2221 tgctgaatcc cctcatctac agcctccgca acaaggaggt gactagagca ttcatgaaga
     2281 tcttaggaaa gggcaagtct gagagtgagt tacctcataa actttatgtt ttgctgtttg
     2341 ctaaattctt ctttctaata tccatctttt tctatgatgt caaaatacta gcattgatta
     2401 tgtacattgc ctaattgcca actttcttgt acaaagttgg cattataaga aagcattgct
     2461 tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt atttgccatc
     2521 cagctgatat cccctatagt gagtcgtatt acatggtcat agctgtttcc tggcagctct
     2581 ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata tcatcatgcc
     2641 tcctctagac cagccaggac agaaatgcct cgacttcgct gctacccaag gttgccgggt
     2701 gacgcacacc gtggaaacgg atgaaggcac gaacccagtg gacataagcc tgttcggttc
     2761 gtaagctgta atgcaagtag cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac
     2821 gcagcggtgg taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttgggg
     2881 tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga
     2941 tgttatggag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaaaca
     3001 ttatgaggga agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca
     3061 tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg
     3121 gcggcctgaa gccacacagt gatattgatt tgctggttac ggtgaccgta aggcttgatg
     3181 aaacaacgcg gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga
     3241 gcgagattct ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc
     3301 gttatccagc taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag
     3361 gtatcttcga gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag
     3421 aacatagcgt tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac
     3481 aggatctatt tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg
     3541 ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg
     3601 gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt
     3661 atcagcccgt catacttgaa gctagacagg cttatcttgg acaagaagaa gatcgcttgg
     3721 cctcgcgcgc agatcagttg gaagaatttg tccactacgt gaaaggcgag atcaccaagg
     3781 tagtcggcaa ataaccctcg agccacccat gaccaaaatc ccttaacgtg agttacg
//
