LOCUS       sequence-166282-        3837 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing OR2M2, part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3837)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 3837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_OR2M2_p.V117I
FEATURES             Location/Qualifiers
     source          1..3837
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      27..615
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     516..535
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     769..786
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(945..972)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1064..1150)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1199..1221
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1213..1230
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1214..1230
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1246..1345
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2393..2492)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2509..2528)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2510..2528)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2533..2549)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2533..2549)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2980..3771
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
       61 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
      121 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
      181 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
      241 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
      301 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
      361 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
      421 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
      481 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
      541 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
      601 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
      661 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
      721 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
      781 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
      841 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
      901 attaatacgc gtaccgctag ccaggaagag tttgtagaaa cgcaaaaagg ccatccgtca
      961 ggatggcctt ctgcttagtt tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc
     1021 ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc ctactcagga
     1081 gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtcttccgac tgagcctttc
     1141 gttttatttg atgcctggca gttccctact ctcgcgttaa cgctagcatg gatgttttcc
     1201 cagtcacgac gttgtaaaac gacggccagt cttaagctcg ggccccaaat aatgatttta
     1261 ttttgactga tagtgacctg ttcgttgcaa caaattgatg agcaatgctt ttttataatg
     1321 ccaactttgt acaaaaaagt tggcaccatg gcatgggaga atcagacctt caactccgac
     1381 ttcatcctcc ttggaatctt caatcacagc ccaccacaca cgttcctctt ctttctggtc
     1441 ctgggcatct ttttagtggc cttcatggga aactctgtca tggttctcct catctacctg
     1501 gacacccagc tccacacccc catgtacttc ctcctcagcc aactgtccct catggacctc
     1561 atgctcatct gcaccaccgt acccaagatg gccttcaact acttgtctgg cagcaagtcc
     1621 atttctatgg ctggttgtgt cacacaaatt ttcttctata tatcactgtc tggctctgaa
     1681 tgttttcttt tggctattat ggcttatgac cgctatattg ctatttgcca ccctctaaga
     1741 tataccaatc tcatgaatcc taaaatttgt ggacttatgg ctaccttctc ctggatcctg
     1801 ggctctacag atggaatcat tgatgctgta gccacatttt ccttctcctt ttgtgggtct
     1861 cgggaaatag cccacttctt ctgtgaattc ccttccctac taatcctctc atgcaatgac
     1921 acatcaatat ttgaagaggt tattttcatc tgctgtatag taatgcttgt tttccctgtt
     1981 gcaatcatca ttgcttccta tgctggagtt attctggctg tcattcacat gggatctgga
     2041 gagggtcgtc gcaaagcttt cacgacctgt tcctctcacc tcatggtggt gggaatgtac
     2101 tatggagcag ctttgttcat gtacatacgg cccacatctg atcactcccc aacgcaggac
     2161 aagatggtgt ctgtattcta caccatcctc actcccatgc tgaatcccct catctacagc
     2221 ctccgcaaca aggaggtgac tagagcattc atgaagatct taggaaaggg caagtctgag
     2281 agtgagttac ctcataaact ttatgttttg ctgtttgcta aattcttctt tctaatatcc
     2341 atctttttct atgatgtcaa aatactagca ttgattatgt acattgccta attgccaact
     2401 ttcttgtaca aagttggcat tataagaaag cattgcttat caatttgttg caacgaacag
     2461 gtcactatca gtcaaaataa aatcattatt tgccatccag ctgatatccc ctatagtgag
     2521 tcgtattaca tggtcatagc tgtttcctgg cagctctggc ccgtgtctca aaatctctga
     2581 tgttacattg cacaagataa aaatatatca tcatgcctcc tctagaccag ccaggacaga
     2641 aatgcctcga cttcgctgct acccaaggtt gccgggtgac gcacaccgtg gaaacggatg
     2701 aaggcacgaa cccagtggac ataagcctgt tcggttcgta agctgtaatg caagtagcgt
     2761 atgcgctcac gcaactggtc cagaaccttg accgaacgca gcggtggtaa cggcgcagtg
     2821 gcggttttca tggcttgtta tgactgtttt tttggggtac agtctatgcc tcgggcatcc
     2881 aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag caacgatgtt
     2941 acgcagcagg gcagtcgccc taaaacaaag ttaaacatta tgagggaagc ggtgatcgcc
     3001 gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg
     3061 ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagtgat
     3121 attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc
     3181 aacgaccttt tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa
     3241 gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg
     3301 caatttggag aatggcagcg caatgacatt cttgcaggta tcttcgagcc agccacgatc
     3361 gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt
     3421 ccagcggcgg aggaactctt tgatccggtt cctgaacagg atctatttga ggcgctaaat
     3481 gaaaccttaa cgctatggaa ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg
     3541 cttacgttgt cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc
     3601 gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat acttgaagct
     3661 agacaggctt atcttggaca agaagaagat cgcttggcct cgcgcgcaga tcagttggaa
     3721 gaatttgtcc actacgtgaa aggcgagatc accaaggtag tcggcaaata accctcgagc
     3781 cacccatgac caaaatccct taacgtgagt tacgcgtcgt tccactgagc gtcagac
//
