LOCUS       sequence-166284-        1221 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of untagged ILID CAAX.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1221)
  AUTHORS   O'Neill PR, Kalyanaraman V, Gautam N
  TITLE     Subcellular optogenetic activation of Cdc42 controls local and 
            distal signaling to drive immune cell migration.
  JOURNAL   Mol Biol Cell. 2016 May 1;27(9):1442-50. doi: 
            10.1091/mbc.E15-12-0832. Epub 2016 Mar 3.
  PUBMED    26941336
REFERENCE   2  (bases 1 to 1221)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E15-12-0832"; journalName: "Mol Biol Cell"; date: 
            "2016-05-1- 1"; volume: "27"; issue: "9"; pages: "1442-50"
COMMENT     Sequence Label: ILID-CAAX
FEATURES             Location/Qualifiers
     source          1..1221
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(122..559)
                     /codon_start=1
                     /product="improved light-inducible dimer when used with 
                     SspB nano or SspB micro (Guntas et al., 2015)"
                     /label=iLID
                     /translation="LATTLERIEKNFVITDPRLPDNPIIFASDSFLQLTEYSREEILGR
                     NCRFLQGPETDRATVRKIRDAIDNQTEVTVQLINYTKSGKKFWNVFHLQPMRDYKGDVQ
                     YFIGVQLDGTERLHGAAEREAVCLIKKTAFQIAEAANDENYF"
     primer_bind     complement(603..622)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(604..622)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(667..869)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(696..716)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 gnnntnacgg ggctctagac tcgagcggcc gccactgtgc tggatatctg cagaattcct
       61 acataattac acactttgtc tttgacttct ttttcttctt tttgctccca ctcccgcttc
      121 caaagtaatt ttcgtcgttc gctgcctcag caatctgaaa ggctgtcttt ttgatcaggc
      181 agaccgcctc acgctcagcg gcgccatgaa gacgttcggt gccatccagc tgcacgccaa
      241 tgaagtactg gacgtcgccc ttataatcgc gcatcggctg caggtggaac acgttccaga
      301 acttcttgcc gctcttggta taattgatca gctgaacggt cacttcagtc tggttgtcaa
      361 tagcatctct gattttccga acagtggcac ggtctgtctc gggtccctgc agaaaacggc
      421 aattgcgccc caggatctct tcccgagaat attctgtcag ctgcaggaag gaatcgctcg
      481 caaaaatgat tgggttgtca ggcagtctcg gatcagtaat cacgaaattt ttctcgatcc
      541 gttccagtgt ggttgccagc atggtaccaa gcttaagttt aaacgctagc cagcttgggt
      601 ctccctatag tgagtcgtat taatttcgat aagccagtaa gcagtgggtt ctctagttag
      661 ccagagagct ctgcttatat agacctccca ccgtacacgc ctaccgccca tttgcgtcaa
      721 tggggcggag ttgttacgac attttggaag tcccgttgat tttggtgcca aaacaaactc
      781 ccattgacgt caatggggtg gagacttgga aatccccgtg agtcaaaccg ctatccacgc
      841 ccattgatgt actgccaaaa ccgcatcacc atggtaatag cgatgactaa tacgtagatg
      901 tactgccaag taaggaaagt cccataaggt catgtactgg gcataatgcc aggcgggcca
      961 tttaccgtca ttgacgtcat agggggcgta cttgggcata tgatacactt gatgtactgc
     1021 cagtgggcag ttaaccgtaa aatacttcca cccattgacg tcatgaaagt cctattggcg
     1081 ttactnnnng nnncntaacg ttcattatgg acgttcaatg gnnnngnncn nnggnnnnnc
     1141 agccaagcgg ccnttaaccg tannnatgtt acgcnnnnnc attangnntn gactangnac
     1201 cgtnnntgat tnnctaatnn a
//
