LOCUS       sequence-16629-e        1369 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1369)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1995
REFERENCE   2  (bases 1 to 1369)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pPD95_57
FEATURES             Location/Qualifiers
     source          1..1369
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             251..271
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(361..383)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 gngttactga tcttactaac taactattct catttaaatt ttcagagctt aaaaatggct
       61 gaaatcactc acaacgatgg atacgctaac aacttggaaa tgaaataagc ttgcatgcct
      121 gcaggtcgac tctagaggat ccccgggatt ggccaaagga cccaaaggta tgtttcgaat
      181 gatactaaca taacatagaa cattttcagg aggacccttg agggtaccga gctcagaaaa
      241 aatgactgct ccaaagaaga agcgtaaggt accggtgggt gaagaccaga aacagcacct
      301 cgaactgagc cgcgatattg cccagcgttt caacgcgctg tatggcgaga tcgatcccgt
      361 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
      421 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
      481 acagttgcga aggtaagttt aaacagatcc atactaacta acttgttctg acataatttt
      541 cagcttgaat ggcgaatggc gctttgcctg gtttccggca ccagaagcgg tgccggaaag
      601 ctggctggag tgcgatcttc ctgaggccga tactgtcgtc gtcccctcaa actggcagat
      661 gcacggttac gatgcgccca tctacaccaa cgtgacctat cccattacgg tcaatccgcc
      721 gtttgttccc acggagaatc cgacgggttg ttactcgctc acatttaatg ttgatgaaag
      781 ctggctacag gaaggccaga cgcgaattat ttttgatggc gttaactcgg cgtttcatct
      841 gtggtgcaac gggcgctggg tcggttacgg ccaggtaagt ttaattaagt tgatactaac
      901 taacaagatc tgattaattt tcaggacagt cgtttgccgt ctgaatttga cctgagcgca
      961 tttttacgcg ccggagaaaa ccgcctcncg gtgatggtgc tgcnctggan tgacggcagt
     1021 tatctggaan atcaggatat gtggcggatg agcggcattt tccgtgacgt ctcgttgctg
     1081 cataaaccga ctacccaatc agcgatttcn tggtgccnct cncttaanga gattcncccn
     1141 ccctgnactg gagntgaagt cnggtanttt taacggnatn tnctactaan tgnctaactg
     1201 antttcnang ggggggggtg nggactcncc gggnannttt ttttggnggg naancagtcc
     1261 ccggcccccc nttnggggaa atncaaagng ggggttgcca tgcccnccct ngagcnaacn
     1321 aannggggcc aacccacttt cngggggtat ccccccnccc ttnnaaacc
//
