LOCUS       sequence-16631-e        1277 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1995
REFERENCE   2  (bases 1 to 1277)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pPD95_67
FEATURES             Location/Qualifiers
     source          1..1277
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             244..264
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(307..335)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 tgatcttcta actaactatt ctcatttaaa ttttcagagc ttaaaaatgg ctgacctcnc
       61 tcacaacgat ggatacgcta acaacttgga aatgaaanaa gcttgcatgc ctgcaggtcg
      121 actctagagg atccccggga ttggccaaag gacccaaagg tatgtttcga atgatactaa
      181 cataacatag aacattttca ggaggaccct tggagggtac cgagctcaga aaaaatgact
      241 gctccaaaga agaagcgtaa ggtaccggta gaaaaaatga gtaaaggaga agaacttttc
      301 actggagttg tcccaattct tgttgaatta gatggtgatg ttaatgggca caaattttct
      361 gtcagtggag agggtgaagg tgatgcaaca tacggaaaac ttacccttaa atttatttgc
      421 actactggaa aactacctgt tccatgggta agtttaaaca tatatatact aactaaccct
      481 gattatttaa attttcagcc aacacttgtc actactttct gttatggtgt tcaatgcttc
      541 tcgagatacc cagatcatat gaaacggcat gactttttca agagtgccat gcccgaaggt
      601 tatgtacagg aaagaactat atttttcaaa gatgacggga actacaagac acgtaagttt
      661 aaacagttcg gtactaacta accatacata tttaaatttt caggtgctga agtcaagttt
      721 gaaggtgata cccttgttaa tagaatcgag ttaaaaggta ttgattttaa agaagatgga
      781 aacattcttg gacacaaatt ggaatacaac tataactcac acaatgtata catcatggca
      841 gacaaacaaa agaatggaat caaagttgta agtttaaaca tgattttact aactaactaa
      901 tctgatttaa ttttcagaac ttcaaattag acacaacatt gaagatggag cgttcactag
      961 cagacnttat cacnaaatac tccattggcg atggcctgtc ctttaccaga caccattact
     1021 gtcccacnat ctgccttcga agatccacga aagnagacnn tggncctctg anttgtacac
     1081 cgcnggatac ctggcnggag actaacaaat agatcnaaat ncactgacnc ggcctcntac
     1141 actgcggggc aaaaaaggnn ngcccnaaag tnacgntccn acacgaantt nttcccnccc
     1201 ncngcngatn agcattctca ncnaggnttc cggcccccct ttttaaaant cttttttttt
     1261 ttttnccccc aaanngg
//
