LOCUS       sequence-166312-        8714 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression of Kv10.2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8714)
  AUTHORS   Gomez-Varela D, Zwick-Wallasch E, Knotgen H, Sanchez A, Hettmann T, 
            Ossipov D, Weseloh R, Contreras-Jurado C, Rothe M, Stuhmer W, Pardo 
            LA
  TITLE     Monoclonal antibody blockade of the human Eag1 potassium channel 
            function exerts antitumor activity.
  JOURNAL   Cancer Res. 2007 Aug 1;67(15):7343-9.
  PUBMED    17671204
REFERENCE   2  (bases 1 to 8714)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Res."; date: "2007-08-1"; volume: "67(15)"; pages: "7343-9"
COMMENT     Sequence Label: pTKv10.2
FEATURES             Location/Qualifiers
     source          1..8714
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4019..4036)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4025..4249
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4295..4723
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4382..4401)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4592..4613
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4732..4752)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4737..5067
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4918..5053
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4980..4999
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        5115..5162
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     primer_bind     complement(5214..5242)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     5837..5857
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             5880..6257
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MDAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRD
                     DVTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWG
                     REFALRDPAGNCVHFVAEEQD"
     polyA_signal    6387..6508
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6424..6443)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6478..6497
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6557..6573)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6557..6573)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6570..6592)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6581..6597
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6605..6611,6612..6629,6630..6635))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6650..6671
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6788..6805)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6959..7547)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7039..7058)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7718..8509,8510..8578))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8341..8360
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8579..8683)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttggta ccgagctcgg atccactagt ccagtgtggt ggaattctgc
      961 agatatccag cacagtggcg gccgcactag tgattctggc cgctgctctc cagacccagg
     1021 atgccggggg gcaagagagg gctggtggca ccgcagaaca catttttgga gaacatcgtc
     1081 aggcgctcca gtgaatcaag tttcttactg ggaaatgccc agattgtgga ttggcctgta
     1141 gtttatagta atgacggttt ttgtaaactc tctggatatc atcgagctga cgtcatgcag
     1201 aaaagcagca cttgcagttt tatgtatggg gaattgactg acaagaagac cattgagaaa
     1261 gtcaggcaaa cttttgacaa ctacgaatca aactgctttg aagttcttct gtacaagaaa
     1321 aacagaaccc ctgtttggtt ttatatgcaa attgcaccaa taagaaatga acatgaaaag
     1381 gtggtcttgt tcctgtgtac tttcaaggat attacgttgt tcaaacagcc aatagaggat
     1441 gattcaacaa aaggttggac gaaatttgcc cgattgacac gggctttgac aaatagccga
     1501 agtgttttgc agcagctcac gccaatgaat aaaacagagg tggtccataa acattcaaga
     1561 ctagctgaag ttcttcagct gggatcagat atccttcctc agtataaaca agaagcgcca
     1621 aagacgccac cacacattat tttacattat tgtgctttta aaactacttg ggattgggtg
     1681 attttaattc ttaccttcta caccgccatt atggttcctt ataatgtttc cttcaaaaca
     1741 aagcagaaca acatagcctg gctggtactg gatagtgtgg tggacgttat ttttctggtt
     1801 gacatcgttt taaattttca cacgactttc gtggggcccg gtggagaggt catttctgac
     1861 cctaagctca taaggatgaa ctatctgaaa acttggtttg tgatcgatct gctgtcttgt
     1921 ttaccttatg acatcatcaa tgcctttgaa aatgtggatg agggaatcag cagtctcttc
     1981 agttctttaa aagtggtgcg tctcttacga ctgggccgtg tggctaggaa actggaccat
     2041 tacctagaat atggagcagc agtcctcgtg ctcctggtgt gtgtgtttgg actggtggcc
     2101 cactggctgg cctgcatatg gtatagcatc ggagactacg aggtcattga tgaagtcact
     2161 aacaccatcc aaatagacag ttggctctac cagctggctt tgagcattgg gactccatat
     2221 cgctacaata ccagtgctgg gatatgggaa ggaggaccca gcaaggattc attgtacgtg
     2281 tcctctctct actttaccat gacaagcctt acaaccatag gatttggaaa catagctcct
     2341 accacagatg tggagaagat gttttcggtg gctatgatga tggttggctc tcttctttat
     2401 gcaactattt ttggaaatgt tacaacaatt ttccagcaaa tgtatgccaa caccaaccga
     2461 taccatgaga tgctgaataa tgtacgggac ttcctaaaac tctatcaggt cccaaaaggc
     2521 cttagtgagc gagtcatgga ttatattgtc tcaacatggt ccatgtcaaa aggcattgat
     2581 acagaaaagg tcctctccat ctgtcccaag gacatgagag ctgatatctg tgttcatcta
     2641 aaccggaagg tttttaatga acatcctgct tttcgattgg ccagcgatgg gtgtctgcgc
     2701 gccttggcgg tagagttcca aaccattcac tgtgctcccg gggacctcat ttaccatgct
     2761 ggagaaagtg tggatgccct ctgctttgtg gtgtcaggat ccttggaagt catccaggat
     2821 gatgaggtgg tggctatttt agggaagggt gatgtatttg gagacatctt ctggaaggaa
     2881 accacccttg cccatgcatg tgcgaacgtc cgggcactga cgtactgtga cctacacatc
     2941 atcaagcggg aagccttgct caaagtcctg gacttttata cagcttttgc aaactccttc
     3001 tcaaggaatc tcactcttac ttgcaatctg aggaaacgga tcatctttcg taagatcagt
     3061 gatgtgaaga aagaggagga ggagcgcctc cggcagaaga atgaggtgac cctcagcatt
     3121 cccgtggacc acccagtcag aaagctcttc cagaagttca agcagcagaa ggagctgcgg
     3181 aatcagggct caacacaggg tgaccctgag aggaaccaac tccaggtaga gagccgctcc
     3241 ttacagaatg gagcctccat caccggaacc agcgtggtga ctgtgtcaca gattactccc
     3301 attcagacgt ctctggccta tgtgaaaacc agtgaatccc ttaagcagaa caaccgtgat
     3361 gccatggaac tcaagcccaa cggcggtgct gaccaaaaat gtctcaaagt caacagccca
     3421 ataagaatga agaatggaaa tggaaaaggg tggctgcgac tcaagaataa tatgggagcc
     3481 catgaggaga aaaaggaaga ctggaataat gtcactaaag ctgagtcaat ggggctattg
     3541 tctgaggacc ccaagagcag tgattcagag aacagtgtga ccaaaaaccc actaaggaaa
     3601 acagattctt gtgacagtgg aattacaaaa agtgaccttc gtttggataa ggctggggag
     3661 gcccgaagtc cgctagagca cagtcccatc caggctgatg ccaagcaccc cttttatccc
     3721 atccccgagc aggccttaca gaccacactg caggaagtca aacacgaact caaagaggac
     3781 atccagctgc tcagctgcag aatgactgcc ctagaaaagc aggtggcaga aattttaaaa
     3841 atactgtcgg aaaaaagcgt accccaggcc tcatctccca aatcccaaat gccactccaa
     3901 gtaccccccc agataccatg tcaggatatt tttagtgtct caaggcctga atcacctgaa
     3961 tctgacaaag atgaaatcca cttttaatct agagggcccg tttaaacccg ctgatcagcc
     4021 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     4081 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
     4141 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
     4201 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg
     4261 gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc
     4321 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     4381 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     4441 ctaaatcggg gcatcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     4501 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     4561 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     4621 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttggggat ttcggcctat
     4681 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt
     4741 gtcagttagg gtgtggaaag tccccaggct ccccaggcag gcagaagtat gcaaagcatg
     4801 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     4861 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     4921 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     4981 atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc
     5041 ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga
     5101 tctgatcagc acgtgttgac aattaatcat cggcatagta tatcggcata gtataatacg
     5161 acaaggtgag gaactaaacc atggctagca aaggagaaga acttttcact ggagttgtcc
     5221 caattcttgt tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg
     5281 gtgaaggtga tgctacatac ggaaagctta cccttaaatt tatttgcact actggaaaac
     5341 tacctgttcc atggccaaca cttgtcacta ctttctctta tggtgttcaa tgcttttccc
     5401 gttatccgga tcatatgaaa cggcatgact ttttcaagag tgccatgccc gaaggttatg
     5461 tacaggaacg cactatatct ttcaaagatg acgggaacta caagacgcgt gctgaagtca
     5521 agtttgaagg tgataccctt gttaatcgta tcgagttaaa aggtattgat tttaaagaag
     5581 atggaaacat tctcggacac aaactcgagt acaactataa ctcacacaat gtatacatca
     5641 cggcagacaa acaaaagaat ggaatcaaag ctaacttcaa aattcgccac aacattgaag
     5701 atggatccgt tcaactagca gaccattatc aacaaaatac tccaattggc gatggccctg
     5761 tccttttacc agacaaccat tacctgtcga cacaatctgc cctttcgaaa gatcccaacg
     5821 aaaagcgtga ccacatggtc cttcttgagt ttgtaactgc tgctgggatt acacatggca
     5881 tggatgccaa gttgaccagt gccgttccgg tgctcaccgc gcgcgacgtc gccggagcgg
     5941 tcgagttctg gaccgaccgg ctcgggttct cccgggactt cgtggaggac gacttcgccg
     6001 gtgtggtccg ggacgacgtg accctgttca tcagcgcggt ccaggaccag gtggtgccgg
     6061 acaacaccct ggcctgggtg tgggtgcgcg gcctggacga gctgtacgcc gagtggtcgg
     6121 aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc catgaccgag atcggcgagc
     6181 agccgtgggg gcgggagttc gccctgcgcg acccggccgg caactgcgtg cacttcgtgg
     6241 ccgaggagca ggactgacac gtgctacgag atttcgattc caccgccgcc ttctatgaaa
     6301 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc
     6361 tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat
     6421 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     6481 gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga
     6541 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     6601 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     6661 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     6721 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     6781 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     6841 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     6901 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     6961 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     7021 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     7081 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     7141 tggcgctttc tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     7201 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     7261 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     7321 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     7381 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
     7441 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     7501 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     7561 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     7621 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     7681 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     7741 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     7801 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     7861 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     7921 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     7981 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     8041 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     8101 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     8161 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     8221 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     8281 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     8341 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     8401 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     8461 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     8521 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     8581 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     8641 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     8701 tgccacctga cgtc
//
