LOCUS       sequence-166314-        5807 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5807)
  AUTHORS   Lee IH, Cao L, Mostoslavsky R, Lombard DB, Liu J, Bruns NE, Tsokos 
            M, Alt FW, Finkel T
  TITLE     A role for the NAD-dependent deacetylase Sirt1 in the regulation of 
            autophagy.
  JOURNAL   Proc Natl Acad Sci U S A. 2008 Mar 4. 105(9):3374-9.
  PUBMED    18296641
REFERENCE   2  (bases 1 to 5807)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2008 Mar 4. 105(9):3374-9."
COMMENT     Sequence Label: pCMV-myc-Atg7
FEATURES             Location/Qualifiers
     source          1..5807
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     201..225
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          342..438
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     polyA_signal    2616..2750
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2640..2659)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2694..2713
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2861..2877)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2861..2877)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2874..2896)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2885..2901
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2909..2915,2916..2933,2934..2939))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2954..2975
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3092..3109)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3263..3851)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3343..3362)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4022..4813,4814..4882))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4645..4664
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4883..4987)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5055..5073
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5111..5133)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5233..5252
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5446..5468
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5460..5477
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5461..5477
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        5504..5807
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat
      241 ccacgctgtt ttgacctcca tagaagacac cgggaccgat ccagcctccg gactctagag
      301 gatccggtac tagaggaact gaaaaaccag aaagttaact ggtaagttta gtctttttgt
      361 cttttatttc aggtcccgga tccggtggtg gtgcaaatca aagaactgct cctcagtgga
      421 tgttgccttt acttctaggc ctgtacggaa gtgttacttc tgctctaaaa gctgcggaat
      481 tgtacccgcg ggcccaccat ggcatcaatg cagaagctga tctcagagga ggacctgctt
      541 atggccatgg aggcccgaat tcaagaaata atggcggcag ctacggggga tcctggactc
      601 tctaaactgc agtttgcccc ttttagtagt gccttggatg ttgggttttg gcatgagttg
      661 acccagaaga agctgaacga gtatcggctg gatgaagctc ccaaggacat taagggttat
      721 tactacaatg gtgactctgc tgggctgcca gctcgcttaa cattggagtt cagtgctttt
      781 gacatgagtg ctcccacccc agcccgttgc tgcccagcta ttggaacact gtataacacc
      841 aacacactcg agtctttcaa gactgcagat aagaagctcc ttttggaaca agcagcaaat
      901 gagatatggg aatccataaa atcaggcact gctcttgaaa accctgtact cctcaacaag
      961 ttcctcctct tgacatttgc agatctaaag aagtaccact tctactattg gttttgctat
     1021 cctgccctct gtcttccaga gagtttacct ctcattcagg ggccagtggg tttggatcaa
     1081 aggttttcac taaaacagat tgaagcacta gagtgtgcat atgataatct ttgtcaaaca
     1141 gaaggagtca cagctcttcc ttacttctta atcaagtatg atgagaacat ggtgctggtt
     1201 tccttgctta aacactacag tgatttcttc caaggtcaaa ggacgaagat aacaattggt
     1261 gtatatgatc cctgtaactt agcccagtac cctggatggc ctttgaggaa ttttttggtc
     1321 ctagcagccc acagatggag tagcagtttc cagtctgttg aagttgtttg cttccgtgac
     1381 cgtaccatgc agggggcgag agacgttgcc cacagcatca tcttcgaagt gaagcttcca
     1441 gaaatggcat ttagcccaga ttgtcctaaa gcagttggat gggaaaagaa ccagaaagga
     1501 ggcatgggac caaggatggt gaacctcagt gaatgtatgg accctaaaag gttagctgag
     1561 tcatcagtgg atctaaatct caaactgatg tgttggagat tggttcctac tttagacttg
     1621 gacaaggttg tgtctgtcaa atgtctgctg cttggagccg gcaccttggg ttgcaatgta
     1681 gctaggacgt tgatgggttg gggcgtgaga cacatcacat ttgtggacaa tgccaagatc
     1741 tcctactcca atcctgtgag gcagcctctc tatgagtttg aagattgcct agggggtggt
     1801 aagcccaagg ctctggcagc agcggaccgg ctccagaaaa tattccccgg tgtgaatgcc
     1861 agaggattca acatgagcat acctatgcct gggcatccag tgaacttctc cagtgtcact
     1921 ctggagcaag cccgcagaga tgtggagcaa ctggagcagc tcatcgaaag ccatgatgtc
     1981 gtcttcctat tgatggacac cagggagagc cggtggcttc ctgccgtcat tgctgcaagc
     2041 aagagaaagc tggtcatcaa tgctgctttg ggatttgaca catttgttgt catgagacat
     2101 ggtctgaaga aaccaaagca gcaaggagct ggggacttgt gtccaaacca ccctgtggca
     2161 tctgctgacc tcctgggctc atcgcttttt gccaacatcc ctggttacaa gcttggctgc
     2221 tacttctgca atgatgtggt ggccccagga gattcaacca gagaccggac cttggaccag
     2281 cagtgcactg tgagtcgtcc aggactggcc gtgattgcag gagccctggc cgtggaattg
     2341 atggtatctg ttttgcagca tccagaaggg ggctatgcca ttgccagcag cagtgacgat
     2401 cggatgaatg agcctccaac ctctcttggg cttgtgcctc accaggttct tgatcaatat
     2461 gaacgagaag gatttaactt cctagccaag gtgtttaatt cttcacattc cttcttagaa
     2521 gacttgactg gtcttacatt gctgcatcaa gaaacccaag ctgctgagat ctgggacatg
     2581 agcgatgatg agaccatctg agatgcggcc gcggggatcc agacatgata agatacattg
     2641 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt
     2701 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca
     2761 attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt tcggatcctc
     2821 tagagtcgat ctgcaggcat gctagcttgg cgtaatcatg gtcatagctg tttcctgtgt
     2881 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     2941 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     3001 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     3061 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     3121 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     3181 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     3241 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     3301 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     3361 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     3421 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     3481 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     3541 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     3601 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     3661 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     3721 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     3781 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     3841 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     3901 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     3961 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     4021 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     4081 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     4141 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     4201 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     4261 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     4321 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     4381 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     4441 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     4501 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     4561 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     4621 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     4681 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     4741 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     4801 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     4861 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     4921 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     4981 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga
     5041 cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg
     5101 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg
     5161 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct
     5221 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa
     5281 taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc
     5341 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag
     5401 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt
     5461 gtaaaacgac ggccagtgag ttcgagcttg catgcctgca ggtcgttaca taacttacgg
     5521 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     5581 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     5641 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
     5701 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     5761 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatg
//
