LOCUS       sequence-16632-e        1216 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1216)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1995
REFERENCE   2  (bases 1 to 1216)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pPD95_69
FEATURES             Location/Qualifiers
     source          1..1216
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             244..264
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(307..335)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 ctgatcttct aactaactat tctcatttaa attttcagag cttaaaaatg gctgaaatca
       61 ctcacaacga tggatacgct aacaacttgg aaatgaaata agcttgcatg cctgcaggtc
      121 gactctagag gatccccggg attggccaaa ggacccaaag gtatgtttcg aatgatacta
      181 acataacata gaacattttc aggaggaccc ttgagggtac cgagctcaga aaaaatgact
      241 gctccaaaga agaagcgtaa ggtaccggta gaaaaaatga gtaaaggaga agaacttttc
      301 actggagttg tcccaattct tgttgaatta gatggtgatg ttaatgggca caaattttct
      361 gtcagtggag agggtgaagg tgatgcaaca tacggaaaac ttacccttaa atttatttgc
      421 actactggaa aactacctgt tccatgggta agtttaaaca tatatatact aactaaccct
      481 gattatttaa attttcagcc aacacttgtc actactttct gttatggtgt tcaatgcttc
      541 tcgagatacc cagatcatat gaaacggcat gactttttca agagtgccat gcccgaaggt
      601 tatgtacagg aaagaactat atttttcaaa gatgacggga actacaagac acgtaagttt
      661 aaacagttcg gtactaacta accatacata tttaaatttt caggtgctga agtcaagttt
      721 gaaggtgata cccttgttaa tagaatcgag ttaaaaggta ttgattttaa agaagatgga
      781 aacattcttg gacacaaatt ggaatacaac tataactcac acaatgtata catcatggca
      841 gacaaacaaa agaatggaat caaagttgta agtttaaaca tgattttact aactaactaa
      901 tctgatttaa attttcagaa cttcaaaatt agacacaaca ttgaagatgg aagcgttcac
      961 ctagcagacc attatcacca aatactccat tggcgatggc cctgtcnttt accagacanc
     1021 nttacctgtc caccaatctg cccttcgaag atcccacgaa aaaaagaccc ntggncttct
     1081 ganttgtacc ctgcnggata cctnggcagg agacttcnaa tagcntcgna antccactga
     1141 ggcgggcctc ctncnactgc cggggcaaaa anaggncngn cncaaantta acggtccncn
     1201 caccnnaatt tattcc
//
